Anti-Hu CD107a Alexa Fluor® 488

Anti-Hu CD107a Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
CD107a
Clone
H4A3
Format
Alexa Fluor® 488
Reactivity
Human, Non-human primates, Mouse
Excitation laser
blue (488 nm)
Variant
100 tests
A4-671-T100
In stock
242.00 USD

25 tests
A4-671-T025
Delivery 1 week
121.00 USD
Variant
100 tests
A4-671-T100
In stock
242.00 USD

25 tests
A4-671-T025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody H4A3 recognizes an extracellular/luminal epitope of CD107a, an approximately 100-120 kDa glycoprotein expressed mainly on lysosomal, but also on the plasma membrane.
Workshop
HLDA V
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular and extracellular staining.
Reactivity
Human, Non-human primates, Mouse
Immunogen
Human PBMC
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
LAMP-1, LAMPA
Antigen description
CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity is observed in neurones, and in glial cells surrounding senile plaques in Alzheimers disease cases and is localized mainly in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.
Entrez Gene ID 3916
UniProt ID P11279
A4-671 FC
Flow cytometry analysis (intracellular staining) of human peripheral blood cells with anti-CD107a (H4A3) Alexa Fluor® 488.

General references:

Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61(1):62-8.
PubMed
Barrachina M, Maes T, Buesa C, Ferrer I: Lysosome-associated membrane protein 1 (LAMP-1) in Alzheimer's disease. Neuropathol Appl Neurobiol. 2006 Oct;32(5):505-16.
PubMed
Sarafian VS, Marinova TT: Lysosomal membrane-associated glycoproteins are differentially expressed in acute and chronic human thymic involution. Acta Biol Hung. 2006 Sep;57(3):315-22.
PubMed
Eskelinen EL: Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Mol Aspects Med. 2006 Oct-Dec;27(5-6):495-502.
PubMed
Hunyh KK, Eskelinen EL, Scott CC, Malevanets A, Saftig P, Grinstein S: LAMP proteins are required for fusion of lysosomes with phagosomes. EMBO J. 2007 Jan 24;26(2):313-24.
PubMed

Product specific references:

Majer F, Vlaskova H, Krol L, Kalina T, Kubanek M, Stolnaya L, Dvorakova L, Elleder M, Sikora J: Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency. Gene. 2012 May 1;498(2):183-95.
PubMed
Mao H, Tu W, Liu Y, Qin G, Zheng J, Chan PL, Lam KT, Peiris JS, Lau YL: Inhibition of human natural killer cell activity by influenza virions and hemagglutinin. J Virol. 2010 May;84(9):4148-57.
PubMed
Yu CI, Gallegos M, Marches F, Zurawski G, Ramilo O, García-Sastre A, Banchereau J, Palucka AK: Broad influenza-specific CD8+ T-cell responses in humanized mice vaccinated with influenza virus vaccines. Blood. 2008 Nov 1;112(9):3671-8.
PubMed
Tomescu C, Chehimi J, Maino VC, Montaner LJ: NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells. J Immunol. 2007 Aug 15;179(4):2097-104.
PubMed
Carlsten M, Björkström NK, Norell H, Bryceson Y, van Hall T, Baumann BC, Hanson M, Schedvins K, Kiessling R, Ljunggren HG, Malmberg KJ: DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells. Cancer Res. 2007 Feb 1;67(3):1317-25.
PubMed
Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths GM, Aricó M, Moretta L, Pende D: Analysis of natural killer-cell function in familial hemophagocytic lymphohistiocytosis (FHL): defective CD107a surface expression heralds Munc13-4 defect and discriminates between genetic subtypes of the disease. Blood. 2006 Oct 1;108(7):2316-23.
PubMed
Deetz CO, Hebbeler AM, Propp NA, Cairo C, Tikhonov I, Pauza CD: Gamma interferon secretion by human Vgamma2Vdelta2 T cells after stimulation with antibody against the T-cell receptor plus the Toll-Like receptor 2 agonist Pam3Cys. Infect Immun. 2006 Aug;74(8):4505-11.
PubMed
Furuta K, Ikeda M, Nakayama Y, Nakamura K, Tanaka M, Hamasaki N, Himeno M, Hamilton SR, August JT: Expression of lysosome-associated membrane proteins in human colorectal neoplasms and inflammatory diseases. Am J Pathol. 2001 Aug;159(2):449-55.
PubMed
Mane SM, Marzella L, Bainton DF, Holt VK, Cha Y, Hildreth JE, August JT: Purification and characterization of human lysosomal membrane glycoproteins. Arch Biochem Biophys. 1989 Jan;268(1):360-78.
PubMed
Variant
100 tests
A4-671-T100
In stock
242.00 USD

25 tests
A4-671-T025
Delivery 1 week
121.00 USD
Variant
100 tests
A4-671-T100
In stock
242.00 USD

25 tests
A4-671-T025
Delivery 1 week
121.00 USD

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