Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD105 Purified

Anti-Hu CD105 Purified

Regulatory status

RUO

Antigen

CD105

Clone

MEM-229

Format

Purified

Reactivity

Pig, Human

Application

WB, FC (QC tested), IHC(F), ICC

Variant

0.1 mg

11-453-C100

In stock

140.00 USD

0.025 mg

11-453-C025

Delivery 1 week

70.00 USD

Variant

0.1 mg

11-453-C100

In stock

140.00 USD

0.025 mg

11-453-C025

Delivery 1 week

70.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody MEM-229 recognizes an extracellular epitope of CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Application

WB, FC (QC tested), IHC(F), ICC

Application details

Flow cytometry: Recommended dilution: 2-6 μg/ml; positive control: Kg1 human acute myelogenous leukemia cell line.
Immunohistochemistry (frozen sections): Recommended dilution: 1:200, acetone fixation.
Western blotting: Non-reducing conditions.

Reactivity

Pig, Human

Negative species

Dog, Horse

Immunogen

Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Endoglin, END, ENG, HHT1, ORW1

Antigen description

CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Entrez Gene ID 2022

UniProt ID P17813

Immunocytochemistry staining of an infarcted porcine heart with anti-CD105 (MEM-229; green); cell nuclei stained with DAPI (blue). Primary antibody dilution: 5 ug/ml.

Separation of HUVEC cells stained using anti-human CD105 (MEM-229) purified antibody (concentration in sample 3 μg/ml, red-filled, GAM APC) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 3 μg/ml, black-dashed, GAM APC) in flow cytometry analysis (surface staining).

General references:

Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34(10):2483-8.
PubMed

Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116(Pt 13):2677-85.
PubMed

Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24(3a):1337-45.
PubMed

Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64.
PubMed

Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13(2):82-9.
PubMed

Product specific references:

Plánka L, Necas A, Srnec R, Rauser P, Starý D, Jancár J, Amler E, Filová E, Hlucilová J, Kren L, Gál P: Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs. Physiol Res. 2009;58(6):885-93.
PubMed