Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD105 PE-Cy™7

Anti-Hu CD105 PE-Cy™7

Regulatory status

RUO

Antigen

CD105

Clone

MEM-226

Format

PE-Cy™7

Reactivity

Rat, Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-298-T100

In stock

275.00 USD

Variant

100 tests

T7-298-T100

In stock

275.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody MEM-226 reacts with an extracellular epitope of CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Rat, Human

Immunogen

Recombinant Vaccinia virus containing the human CD105 cDNA.

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

Endoglin, END, ENG, HHT1, ORW1

Antigen description

CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Entrez Gene ID 2022

UniProt ID P17813

Flow cytometry surface staining pattern of HUVEC cell suspension stained using anti-human CD105 (MEM-226) PE-Cy™7 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Separation of HUVEC cells (red-filled) from HEK-293 cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human CD105 (MEM-226) PE-Cy™7 antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

General references:

Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct;34(10):2483-8.
PubMed

Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1;116(Pt 13):2677-85.
PubMed

Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor-beta-Smad3 signalling. Anticancer Res. 2004 May-Jun;24(3a):1337-45.
PubMed

Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun;25(3B):1851-64.
PubMed

Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr;13(2):82-9.
PubMed

Product specific references:

Schmidt D, Achermann J, Odermatt B, Breymann C, Mol A, Genoni M, Zund G, Hoerstrup SP: Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source. Circulation. 2007 Sep 11;116(11 Suppl):I64-70.
PubMed

Asumda FZ, Chase PB: Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol. 2011 Oct 12;12:44.
PubMed

Liang L, Zheng D, Lu C, Xi Q, Bao H, Li W, Gu Y, Mao Y, Xu B, Gu X: Exosomes derived from miR-301a-3p-overexpressing adipose-derived mesenchymal stem cells reverse hypoxia-induced erectile dysfunction in rat models. Stem Cell Res Ther. 2021 Jan 25;12(1):87.
PubMed

Zhang J, Tang J, Liu J, Yan B, Yan B, Huang M, Zhang Z, Wang L: Melatonin promotes heterotopic ossification through regulation of endothelial-mesenchymal transition in injured achilles tendons in rats. Front Cell Dev Biol. 2021 Feb 11;9:629274.
PubMed

Chang Y, Ben Y, Li H, Xiong Y, Chen G, Hao J, Ma X, Gao X, Qiang P, Shimosawa T, Wang X, Yang F, Xu Q: Eplerenone prevents cardiac fibrosis by inhibiting angiogenesis in unilateral urinary obstruction rats. J Renin Angiotensin Aldosterone Syst. 2022 Sep 17;2022:1283729.
PubMed