Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD103 Purified

Anti-Hu CD103 Purified

Regulatory status

RUO

Antigen

CD103

Clone

Ber-ACT8

Format

Purified

Reactivity

Human, Non-human primates

Application

IP, WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-670-C100

In stock

130.00 USD

0.025 mg

11-670-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-261-C100

In stock

130.00 USD

0.1 mg

11-261-C100

In stock

65.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.

Workshop

HLDA V: WS Code A067

Application

IP, WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human, Non-human primates

Immunogen

HTLV-1 induced human T cell line MAPS16

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Integrin alpha E, ITGAE

Antigen description

CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.

Entrez Gene ID 3682

UniProt ID P38570

Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Product specific references:

Poles MA, Barsoum S, Yu W, Yu J, Sun P, Daly J, He T, Mehandru S, Talal A, Markowitz M, Hurley A, Ho D, Zhang L: Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy. J Virol. 2003 Oct;77(19):10456-67.
PubMed

Jaensson E, Uronen-Hansson H, Pabst O, Eksteen B, Tian J, Coombes JL, Berg PL, Davidsson T, Powrie F, Johansson-Lindbom B, Agace WW: Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J Exp Med. 2008 Sep 1;205(9):2139-49.
PubMed

Zhang L, Bertucci AM, Ramsey-Goldman R, Burt RK, Datta SK: Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of lupus. J Immunol. 2009 Nov 15;183(10):6346-58.
PubMed

Cardoso CR, Garlet GP, Moreira AP, Júnior WM, Rossi MA, Silva JS: Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis. J Leukoc Biol. 2008 Jul;84(1):311-8.
PubMed

Brandes M, Willimann K, Lang AB, Nam KH, Jin C, Brenner MB, Morita CT, Moser B: Flexible migration program regulates gamma delta T-cell involvement in humoral immunity. Blood. 2003 Nov 15;102(10):3693-701.
PubMed

Kost CB, Holden JT, Mann KP: Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep;74(5):282-6.
PubMed

French JJ, Cresswell J, Wong WK, Seymour K, Charnley RM, Kirby JA: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy? Br J Cancer. 2002 Oct 21;87(9):1034-41.
PubMed

Sigmundsdottir H, Johnston A, Gudjonsson JE, Bjarnason B, Valdimarsson H: Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin. Exp Dermatol. 2004 Jul;13(7):426-34.
PubMed

León F, Roy G: Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads. Lab Invest. 2004 Jun;84(6):804-9.
PubMed

Variant

0.1 mg

11-670-C100

In stock

130.00 USD

0.025 mg

11-670-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-261-C100