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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Hu CD103 PE-Cy™7
Anti-Hu CD103 PE-Cy™7
Regulatory status
RUO
Antigen
CD103
Clone
Ber-ACT8
Format
PE-Cy™7
Reactivity
Human, Non-human primates
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
T7-670-T100
In stock
275.00 USD
Variant
100 tests
T7-670-T100
In stock
275.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.
Workshop
HLDA V: WS Code A067
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10
6
cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Immunogen
HTLV-1 induced human T cell line MAPS16
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
Integrin alpha E, ITGAE
Antigen description
CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.
Entrez Gene ID
3682
UniProt ID
P38570
Flow cytometry surface staining pattern of PMA stimulated human peripheral blood mononuclear cells stained using anti-human CD103 (Ber-ACT8) PE-Cy™7 antibody (concentration in sample 0.33 μg/ml).
Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of PMA stimulated human peripheral blood mononuclear cells stained using anti-human CD103 (Ber-ACT8) PE-Cy™7 antibody (concentration in sample 0.33 μg/ml).
Product specific references:
Poles MA, Barsoum S, Yu W, Yu J, Sun P, Daly J, He T, Mehandru S, Talal A, Markowitz M, Hurley A, Ho D, Zhang L: Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy. J Virol. 2003 Oct;77(19):10456-67.
PubMed
Jaensson E, Uronen-Hansson H, Pabst O, Eksteen B, Tian J, Coombes JL, Berg PL, Davidsson T, Powrie F, Johansson-Lindbom B, Agace WW: Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. J Exp Med. 2008 Sep 1;205(9):2139-49.
PubMed
Zhang L, Bertucci AM, Ramsey-Goldman R, Burt RK, Datta SK: Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of lupus. J Immunol. 2009 Nov 15;183(10):6346-58.
PubMed
Cardoso CR, Garlet GP, Moreira AP, Júnior WM, Rossi MA, Silva JS: Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis. J Leukoc Biol. 2008 Jul;84(1):311-8.
PubMed
Brandes M, Willimann K, Lang AB, Nam KH, Jin C, Brenner MB, Morita CT, Moser B: Flexible migration program regulates gamma delta T-cell involvement in humoral immunity. Blood. 2003 Nov 15;102(10):3693-701.
PubMed
Kost CB, Holden JT, Mann KP: Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis. Cytometry B Clin Cytom. 2008 Sep;74(5):282-6.
PubMed
French JJ, Cresswell J, Wong WK, Seymour K, Charnley RM, Kirby JA: T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy? Br J Cancer. 2002 Oct 21;87(9):1034-41.
PubMed
Sigmundsdottir H, Johnston A, Gudjonsson JE, Bjarnason B, Valdimarsson H: Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin. Exp Dermatol. 2004 Jul;13(7):426-34.
PubMed
León F, Roy G: Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads. Lab Invest. 2004 Jun;84(6):804-9.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
100 tests
T7-670-T100
In stock
275.00 USD
Variant
100 tests
T7-670-T100
In stock
275.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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