Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD101 Purified

Anti-Hu CD101 Purified

Regulatory status

RUO

Antigen

CD101

Clone

BB27

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), IHC(F)

Variant

0.1 mg

11-778-C100

In stock

130.00 USD

0.025 mg

11-778-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-778-C100

In stock

130.00 USD

0.025 mg

11-778-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody BB27 recognizes an extracellular epitope of CD101, a 140 kDa disulfide-bonded homodimeric protein expressed on activated T cells, and some other cell types, such as granulocytes and cells of the monocyte/macropgage lineage.

Workshop

HLDA V: WS Code T040

Application

IP, WB, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Human thymic clone B12

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IGSF2, V7, EWI-101

Antigen description

CD101 is a type I transmembrane glycoprotein, which forms disulfide-linked homodimers. It is expressed on activated T cells, as well as on granulocytes, monocytes, dendritic cells or mucosal T cells. It plays a major role in the activation of T cells by skin dendritic cells. Function of CD101 has not been fully elucidated, but in mice its knock-out results in liver autoimmune disease induced by Novosphingobium aromaticivorans.

Entrez Gene ID 9398

UniProt ID Q93033

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD101 (BB27) purified antibody (concentration in sample 0,56 μg/ml, GAM FITC).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD101 (BB27) purified antibody (concentration in sample 0,56 μg/ml, GAM FITC) and anti-human CD16 (3G8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD101 positive CD16 negative lymphocytes (red-filled) CD101 negative CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD101 (BB27) purified antibody (concentration in sample 0,56 μg/ml, GAM FITC).

Product specific references:

Bagot M, Martinel I, Charue D, Boulland ML, Wechsler J, Bensussan A, Boumsell L: Functional role of CD101 on skin dendritic cells. Adv Exp Med Biol. 1997;417:227-32.
PubMed

Grassi F, Dezutter-Dambuyant C, McIlroy D, Jacquet C, Yoneda K, Imamura S, Boumsell L, Schmitt D, Autran B, Debré P, Hosmalin A: Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol. 1998 Oct;64(4):484-93.
PubMed

Variant

0.1 mg

11-778-C100

In stock

130.00 USD

0.025 mg

11-778-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-778-C100