Anti-Hu CD100 Purified

Anti-Hu CD100 Purified
Regulatory status
RUO
Antigen
CD100
Clone
133-1C6
Format
Purified
Reactivity
Human
Application
ELISA, IP, FC (QC tested)
Variant
0.1 mg
11-772-C100
In stock
130.00 USD

0.025 mg
11-772-C025
Ask for delivery term HERE
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The mouse monoclonal antibody 133-1C6 recognizes an extracellular epitope of CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.
Workshop
HLDA VI: WS Code N-L026
Application
ELISA, IP, FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
PHA stimulated human PBL
Other names
COLL4, SEMA4D, semaphorin 4D
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
COLL4, SEMA4D, semaphorin 4D
Antigen description
CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.
Entrez Gene ID 10507
UniProt ID Q92854
11-772 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD100 (133-1C6) purified, GAM-APC.

Product specific references:

Jiang X, Björkström NK, Melum E: Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation. Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation. Front Immunol. 2017 Jun 30;8:765. doi: 10.3389/fimmu.2017.00765.
PubMed
Variant
0.1 mg
11-772-C100
In stock
130.00 USD

0.025 mg
11-772-C025
Ask for delivery term HERE
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD

0.1 mg
11-261-C100
In stock
65.00 USD