Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD100 Purified

Anti-Hu CD100 Purified

Regulatory status

RUO

Antigen

CD100

Clone

133-1C6

Format

Purified

Reactivity

Human

Application

ELISA, IP, FC (QC tested)

Variant

0.1 mg

11-772-C100

In stock

147.40 USD

0.025 mg

11-772-C025

Delivery 1 week

73.70 USD

Variant

0.1 mg

11-772-C100

In stock

147.40 USD

0.025 mg

11-772-C025

Delivery 1 week

73.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody 133-1C6 recognizes an extracellular epitope of CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.

Workshop

HLDA VI: WS Code N-L026

Application

ELISA, IP, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

PHA stimulated human PBL

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

COLL4, SEMA4D, semaphorin 4D

Antigen description

CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.

Entrez Gene ID 10507

UniProt ID Q92854

Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD100 (133-1C6) purified, GAM-APC.

Product specific references:

Jiang X, Björkström NK, Melum E: Intact CD100-CD72 interaction necessary for TCR-induced T cell proliferation. Front Immunol. 2017 Jun 30;8:765. doi: 10.3389/fimmu.2017.00765.
PubMed