Anti-Hu CD100 PE

Anti-Hu CD100 PE
Regulatory status
RUO
Antigen
CD100
Clone
133-1C6
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-772-T100
In stock
220.00 USD

25 tests
1P-772-T025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The mouse monoclonal antibody 133-1C6 recognizes an extracellular epitope of CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.
Workshop
HLDA VI: WS Code N-L026
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
PHA stimulated human PBL
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
COLL4, SEMA4D, semaphorin 4D
Antigen description
CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.
Entrez Gene ID 10507
UniProt ID Q92854
1P-772 FC
Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD100 (133-1C6) PE.

Product specific references:

Jiang X, Björkström NK, Melum E: Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation. Intact CD100-CD72 Interaction Necessary for TCR-Induced T Cell Proliferation. Front Immunol. 2017 Jun 30;8:765. doi: 10.3389/fimmu.2017.00765.
PubMed
Variant
100 tests
1P-772-T100
In stock
220.00 USD

25 tests
1P-772-T025
Delivery 1 week
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD