Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD100 FITC

Anti-Hu CD100 FITC

Regulatory status

RUO

Antigen

CD100

Clone

133-1C6

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-772-T100

In stock

198.00 USD

Variant

100 tests

1F-772-T100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody 133-1C6 recognizes an extracellular epitope of CD100, an approximately 150 kDa (when reduced) semaphorin family member expressed mainly on lymphocytes, NK cells, monocytes/macrophages and granulocytes, but also on some non-hematopoietic cells.

Workshop

HLDA VI: WS Code N-L026

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

PHA stimulated human PBL

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

COLL4, SEMA4D, semaphorin 4D

Antigen description

CD100, also known as semaphorin 4D, is a homodimerizing type I transmembrane glycoprotein containing an extracellular semaphorin domain. It is expressed on most hematopoietic cells with the exception of immature bone marrow cells, erythrocytes and platelets. A 120 kDa soluble form is generated from the transmembrane form by proteolytic cascade following primary T and B cell activation. It seems CD100 acts through dampening CD72-mediated negative signaling. CD100 promotes angiogenesis, invasive growth, proliferation and anti-apoptosis of cancer cells in vitro. Higher expression levels of CD100 correlate with poor survival in soft tissue sarcoma patients.

Entrez Gene ID 10507

UniProt ID Q92854

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD100 (133-1C6) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD100 (133-1C6) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD100 positive CD56 positive lymphocytes (red-filled) from CD100 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD100 (133-1C6) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Jiang X, Björkström NK, Melum E: Intact CD100-CD72 interaction necessary for TCR-induced T cell proliferation. Front Immunol. 2017 Jun 30;8:765. doi: 10.3389/fimmu.2017.00765.
PubMed