Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD10 FITC

Anti-Hu CD10 FITC

Regulatory status

RUO

Antigen

CD10

Clone

LT10

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1F-413-T100

In stock

180.00 USD

25 tests

1F-413-T025

Delivery 1 week

90.00 USD

Variant

100 tests

1F-413-T100

In stock

180.00 USD

25 tests

1F-413-T025

Delivery 1 week

90.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

The antibody LT10 reacts with an extracellular epitope of CD10 (CALLA - Common acute lymphatic leukemia antigen), a 100 kDa type II integral membrane protein.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

mouse NALM-6 leukemia pre-B cell line (tissue/cell preparation)

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CALLA, Neprilysin, Neutral endopeptidase, Enkephalinase, Atriopeptidase, MME

Antigen description

CD10 (neutral endopeptidase – NEP, common acute lymphocytic leukemia antigen – CALLA, membrane metallo-endopeptidase – MME, enkefalinase) is a 100-kDa cell surface zinc metalloprotease, cleaving peptide bonds on the N-terminus of hydrophobic amino acids and inactivating multiple physiologically active peptids. CD10 is expressed on various normal cell types, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelial cells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10 is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiation pathway and has a key role in regulation of growth, differentiation and signal transduction of many cellular systems.

Entrez Gene ID 4311

UniProt ID P08473

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD10 (LT10) FITC (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD10 positive neutrophil granulocytes (red-filled) from human CD10 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD10 (LT10) FITC (20 μl reagent / 100 μl of peripheral whole blood).

General references:

Suzuki T, Ino K, Kikkawa F, Uehara C, Kajiyama H, Shibata K, Mizutani S: Neutral endopeptidase/CD10 expression during phorbol ester-induced differentiation of choriocarcinoma cells through the protein kinase C- and extracellular signal-regulated kinase-dependent signalling pathway. Placenta. 2002 Jul;23(6):475-82.
PubMed

Yada K, Kashima K, Daa T, Kitano S, Fujiwara S, Yokoyama S: Expression of CD10 in basal cell carcinoma. Am J Dermatopathol. 2004 Dec;26(6):463-71.
PubMed

Braham H, Trimeche M, Ziadi S, Mestiri S, Mokni M, Amara K, Hachana M, Sriha B, Korbi S: CD10 expression by fusiform stromal cells in nasopharyngeal carcinoma correlates with tumor progression. Virchows Arch. 2006 Aug;449(2):220-4.
PubMed

Dall'Era MA, True LD, Siegel AF, Porter MP, Sherertz TM, Liu AY: Differential expression of CD10 in prostate cancer and its clinical implication. BMC Urol. 2007 Mar 2;7:3.
PubMed

Variant

100 tests

1F-413-T100

In stock

180.00 USD

25 tests

1F-413-T025

Delivery 1 week

90.00 USD

Variant

100 tests

1F-413-T100