Anti-Hu C5aR2 Purified

Anti-Hu C5aR2 Purified
Regulatory status
RUO
Antigen
C5aR2
Clone
1D9-M12
Format
Purified
Reactivity
Human, Non-human primates
Application
WB, FC (QC tested)
Variant
0.1 mg
11-880-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody 1D9-M12 recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages.
Application
WB, FC (QC tested)
Reactivity
Human, Non-human primates
Immunogen
L1.2 cells transfected with human C5aR2
Other names
C5L2, GPR77, GPF77
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-880_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C5aR2 (1D9-M12) Purified antibody (concentration in sample 5,0 μg/ml, GAM APC).
11-880_FC_Histogram
Separation of monocytes stained using anti-C5aR2 (1D9-M12) purified antibody (concentration in sample 5,0 μg/ml, GAM-APC, red-filled) from monocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Muenstermann M, Strobel L, Klos A, Wetsel RA, Woodruff TM, Köhl J, Johswich KO: Distinct roles of the anaphylatoxin receptors C3aR, C5aR1 and C5aR2 in experimental meningococcal infections. Virulence 2019 Dec;10(1):677-694.
PubMed
Fonseca MI, McGuire SO, Counts SE, Tenner AJ: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology. J Neuroinflammation 2013, Feb 8;10:25
PubMed
An LL, Gorman JV, Stephens G, Swerdlow B, Warrener P, Bonnell J, Mustelin T, Fung M, Kolbeck R: Complement C5a induces PD-L1 expression and acts in synergy with LPS through Erk1/2 and JNK signaling pathways. Sci Rep. 2016 Sep 14;6:33346.
PubMed
Spaan AN, Vrieling M, Wallet P, Badiou C, Reyes-Robles T, Ohneck EA, Benito Y, de Haas CJ, Day CJ, Jennings MP, Lina G, Vandenesch F, van Kessel KP, Torres VJ, van Strijp JA, Henry T: The staphylococcal toxins γ-haemolysin AB and CB differentially target phagocytes by employing specific chemokine receptors. Nat Commun. 2014 Nov 11;5:5438.
PubMed
Hao J, Wang C, Yuan J, Chen M, Zhao MH: A pro-inflammatory role of C5L2 in C5a-primed neutrophils for ANCA-induced activation . PLoS One, 2013, Jun 13;8(6):e66305.
PubMed
Variant
0.1 mg
11-880-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD