Anti-Hu C3aR Purified

Anti-Hu C3aR Purified
Regulatory status
RUO
Antigen
C3aR
Clone
HC3aRZ8
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-882-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
Human C3aR transfectants
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
AZ3B, HNFAG09
Antigen description
C3aR is a 7TM transmembrane protein associated with G proteins, and serves as a receptor for C3a complement fragment. It is expressed mainly on neutrophils, mast cells, basophils, eosinophils, dendritic cells, monocytes, and macrophages. Upon detection of its ligand, the activated C3aR signaling cascade results in degranulation, superoxide production, and chemotaxis.
Entrez Gene ID 719
UniProt ID Q16581
11-882_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).
11-882_FC_Histogram
Separation of human neutrophil granulocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human C3aR (HC3aRZ8) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Product specific references:

Abdelbaset-Ismail A, Borkowska-Rzeszotek S, Kubis E, Bujko K, Brzeźniakiewicz-Janus K, Bolkun L, Kloczko J, Moniuszko M, Basak GW, Wiktor-Jedrzejczak W, Ratajczak MZ: Activation of the complement cascade enhances motility of leukemic cells by downregulating expression of HO-1. Leukemia 2017 Feb;31(2):446-458.
PubMed
Larsen LF, Juel-Berg N, Hansen A, Hansen KS, Mills EN, van Ree R, Rådinger M, Poulsen LK, Jensen BM: No difference in human mast cells derived from peanut allergic versus non-allergic subjects. Immun Inflamm Dis. 2018 Dec;6(4):416-427
PubMed
Reis ES, Chen H, Sfyroera G, Monk PN, Köhl J, Ricklin D, Lambris JD: C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a: insights from a novel label-free cellular assay. J Immunol. 2012 Nov 15;189(10):4797-805.
PubMed
Soruri A, Kiafard Z, Dettmer C, Riggert J, Köhl J, Zwirner J: IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo. J Immunol. 2003 Mar 15;170(6):3306-14.
PubMed
Kiafard Z, Tschernig T, Schweyer S, Bley A, Neumann D, Zwirner J: Use of monoclonal antibodies to assess expression of anaphylatoxin receptors in tubular epithelial cells of human, murine and rat kidneys. Immunobiology 2007;212(2):129-39.
PubMed
Variant
0.1 mg
11-882-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD