Anti-Granzyme B FITC

Anti-Granzyme B FITC
Regulatory status
RUO
Antigen
Granzyme B
Clone
CLB-GB11
Format
FITC
Reactivity
Non-human primates, Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1F-634-T100
In stock
360.00 USD
Variant
0.1 mg
11-261-C100
In stock
360.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.
Reactivity
Non-human primates, Human
Immunogen
Human NK cell line YT-INDY-derived granzyme B
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
GZMB, HLP, CTLA1, SECT
Antigen description
Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.
Entrez Gene ID 3002
UniProt ID P10144
1F-634_FC_Profil značení
Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
1F-634_FC_Dot-plot
Flow cytometry multicolor intracellular staining of human lymphocytes stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1F-634_FC_Histogram
Separation of human CD3 negative Granzyme B positive lymphocytes (red-filled) from CD3 negative Granzyme B negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Granzyme B (CLB-GB11) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

Martin P, Pardo J, Schill N, Joeckel L, Berg M, Froelich CJ, Wallich R, Simon MM: Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics. J Biol Chem. 2010 Apr 15. [Epub ahead of print]
PubMed
Kummer JA, Kamp AM, Tadema TM, Vos W, Meijer CJ, Hack CE: Localization and identification of granzymes A and B-expressing cells in normal human lymphoid tissue and peripheral blood. Clin Exp Immunol. 1995 Apr;100(1):164-72.
PubMed
Kummer JA, Wever PC, Kamp AM, ten Berge IJ, Hack CE, Weening JJ: Expression of granzyme A and B proteins by cytotoxic lymphocytes involved in acute renal allograft rejection. Kidney Int. 1995 Jan;47(1):70-7.
PubMed

Product specific references:

Tak PP, Spaeny-Dekking L, Kraan MC, Breedveld FC, Froelich CJ, Hack CE: The levels of soluble granzyme A and B are elevated in plasma and synovial fluid of patients with rheumatoid arthritis (RA). Clin Exp Immunol. 1999 May;116(2):366-70.
PubMed
Rissoan MC, Duhen T, Bridon JM, Bendriss-Vermare N, Péronne C, de Saint Vis B, Brière F, Bates EE: Subtractive hybridization reveals the expression of immunoglobulin-like transcript 7, Eph-B1, granzyme B, and 3 novel transcripts in human plasmacytoid dendritic cells. Blood. 2002 Nov 1;100(9):3295-303.
PubMed
Sun Y, Schmitz JE, Acierno PM, Santra S, Subbramanian RA, Barouch DH, Gorgone DA, Lifton MA, Beaudry KR, Manson K, Philippon V, Xu L, Maecker HT, Mascola JR, Panicali D, Nabel GJ, Letvin NL: Dysfunction of simian immunodeficiency virus/simian human immunodeficiency virus-induced IL-2 expression by central memory CD4+ T lymphocytes. J Immunol. 2005 Apr 15;174(8):4753-60.
PubMed
Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer JA, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE: Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998 Apr 1;160(7):3610-6.
PubMed
Variant
100 tests
1F-634-T100
In stock
360.00 USD
Variant
0.1 mg
11-261-C100
In stock
360.00 USD