Anti-GABA(B) R1a Purified

Anti-GABA(B) R1a Purified
Regulatory status
RUO
Antigen
GABA(B) R1a
Clone
PAb (549)
Format
Purified
Reactivity
Mouse, Rat
Application
Variant
0.1 mg
11-549-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Product details
Description
References
Isotype
Rabbit polyclonal
Specificity
The polyclonal antibody recognizes N-terminus of mouse gamma-aminobutyric acid (GABA) B receptor 1a. GB1a apparent MW ~130 kDa.
Application
Reactivity
Mouse, Rat
Immunogen
Synthetic peptide (coupled with THG) derived from aminoacid sequence 100-124 of mouse GABA B receptor 1. The sequence has 100% homology with rat and human.
Concentration
1 mg/ml
Preparation
Purified from rabbit serum by precipitation methods
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
GABABR1a, GABBR1a
Antigen description
GABA B receptor is a G-protein-coupled inhibitory receptor of gamma-aminobutyric acid (GABA), and has important functions in brain by inhibition of adenylyl cyclase and modulation of G-protein-gated Ca<sup>2+</sup> and K<sup>+</sup> channels. GABA B receptor is comprised of two subunits, GB1 and GB2 with N-terminal extracellular and C-terminal intracellular domains. The GB1 subunit plays a critical role in ligand binding, whereas the GB2 subunit contains the determinants required for G-protein signaling. Multiple allosteric interactions between the two subunits are required for correct functioning of the receptor. There are two N-terminal splice variants of GB1 subunit, termed GB1a and GB1b; their expression in the central nervous system changes during the ontogenesis and differs between various regions of the brain.
Entrez Gene ID 81657
UniProt ID Q9Z0U4

General references:

Kniazeff J, Galvez T, Labesse G, Pin JP: No ligand binding in the GB2 subunit of the GABA(B) receptor is required for activation and allosteric interaction between the subunits. J Neurosci. 2002 Sep 1;22(17):7352-61.
PubMed
Margeta-Mitrovic M, Jan YN, Jan LY: Function of GB1 and GB2 subunits in G protein coupling of GABA(B) receptors. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14649-54.
PubMed
Margeta-Mitrovic M, Jan YN, Jan LY: Ligand-induced signal transduction within heterodimeric GABA(B) receptor. Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14643-8.
PubMed
Galvez T, Duthey B, Kniazeff J, Blahoš J, Rovelli G, Bettler B, Prézeau L, Pin JP: Allosteric interactions between GB1 and GB2 subunits are required for optimal GABA(B) receptor function. EMBO J. 2001 May 1;20(9):2152-9.
PubMed
Fritschy JM, Meskenaite V, Weinmann O, Honer M, Benke D, Mohler H: GABAB-receptor splice variants GB1a and GB1b in rat brain: developmental regulation, cellular distribution and extrasynaptic localization. Eur J Neurosci. 1999 Mar;11(3):761-8.
PubMed
Variant
0.1 mg
11-549-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD