Exbio — Research products — Antibodies — CD and related antigens — Anti-FOLR2 Purified

Anti-FOLR2 Purified

Regulatory status

RUO

Antigen

FOLR2

Clone

EM-35

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested), ICC

Variant

0.1 mg

11-696-C100

In stock

143.00 USD

0.025 mg

11-696-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-696-C100

In stock

143.00 USD

0.025 mg

11-696-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody EM-35 recognizes an extracellular epitope on FOLR2, a 30-40 kDa cell surface protein serving as a receptor for folic acid.

Application

IP, WB, FC (QC tested), ICC

Application details

Western blotting: Non-reducing conditions.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Negative species

Mouse

Immunogen

BW5147alpha,beta- cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Folate receptor beta, FBP, FR-P3, FR-BETA, BETA-HFR, FBP/PL-1

Antigen description

FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.

Entrez Gene ID 2350

UniProt ID P14207

Western blotting analysis of FOLR2 in THP1 cells (A) and FOLR2-transfected THP1 cells (B) using anti-FOLR2 (EM-35) purified.

Separation of FORL2 transfected BW5147 cells stained using anti-FOLR2 (EM-35) purified antibody (concentration in sample 4 μg/ml, GAM APC, red-filled) from FORL2 transfekted BW5147 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38.
PubMed

Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852.
PubMed