Exbio — Research products — Antibodies — CD and related antigens — Anti-FOLR2 PE

Anti-FOLR2 PE

Regulatory status

RUO

Antigen

FOLR2

Clone

EM-35

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-696-C100

In stock

242.00 USD

0.025 mg

1P-696-C025

Delivery 1 week

121.00 USD

Variant

0.1 mg

1P-696-C100

In stock

242.00 USD

0.025 mg

1P-696-C025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody EM-35 recognizes an extracellular epitope on FOLR2, a 30-40 kDa cell surface protein serving as a receptor for folic acid.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human

Negative species

Mouse

Immunogen

BW5147alpha,beta- cells

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Folate receptor beta, FBP, FR-P3, FR-BETA, BETA-HFR, FBP/PL-1

Antigen description

FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.

Entrez Gene ID 2350

UniProt ID P14207

Separation of cells stained using anti-human FOLR2 (EM-35) PE antibody (concentration in sample 5 μg/ml, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as FORL2 PE antibody concentration, black-dashed) in flow cytometry analysis (surface staining) of FOLR2 transfected BW5147 cell suspension.

Product specific references:

Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38.
PubMed

Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852.
PubMed