Exbio — Research products — Antibodies — CD and related antigens — Anti-FOLR2 Alexa Fluor<sup>®</sup> 647

Anti-FOLR2 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

FOLR2

Clone

EM-35

Format

Alexa Fluor^® 647

Reactivity

Human

Application

WB, FC (QC tested), ICC

Excitation laser

red (633 nm)

Variant

0.1 mg

A6-696-C100

In stock

242.00 USD

Variant

0.1 mg

A6-696-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody EM-35 recognizes an extracellular epitope on FOLR2, a 30-40 kDa cell surface protein serving as a receptor for folic acid.

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 0.2-4 μg/ml.

Reactivity

Human

Negative species

Mouse

Immunogen

BW5147alpha,beta- cells

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Folate receptor beta, FBP, FR-P3, FR-BETA, BETA-HFR, FBP/PL-1

Antigen description

FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.

Entrez Gene ID 2350

UniProt ID P14207

Separation of cells stained using anti-FOLR2 (EM-35) Alexa Fluor® 647 antibody (concentration in sample 0.2 μg/ml, red-filled) from cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 647 antibody (concentration in sample 0.2 μg/ml, same as FOLR2 Alexa Fluor® 647 concentration, black-dashed) in flow cytometry analysis (surface staining) of FOLR2 transfected BW5147 cell suspension.

Product specific references:

Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15;197(6):2229-38.
PubMed

Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 27;9:852.
PubMed