Exbio — Research products — Antibodies — CD and related antigens — Anti-CDK1 Purified

Anti-CDK1 Purified

Regulatory status

RUO

Antigen

CDK1

Clone

POH-1

Format

Purified

Reactivity

Cow, Non-human primates, Human

Application

IP, WB, IHC-P, ICC

Variant

0.1 mg

11-115-C100

In stock

165.00 USD

0.025 mg

11-115-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-115-C100

In stock

165.00 USD

0.025 mg

11-115-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The antibody POH-1 specifically detects Cdk1 (p34Cdc2; an intracellular antigen). Staining of Cdk1 reflects the proliferating potential of respective tissue.

Application

IP, WB, IHC-P, ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: thymus.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Cow, Non-human primates, Human

Negative species

Mouse, Drosophila, Rat, Xenopus

Immunogen

Bacterially expressed full-length human Cdk1 protein

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

p34Cdc2, p34 protein kinase, CDK1, CDC2, CDC28A

Antigen description

Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.

Entrez Gene ID 983

UniProt ID B7Z3D6

Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (clone POH-1). Commercially tested by LifeSpan BioSciences.

Western blotting analysis of human Cdk1 using mouse monoclonal antibody POH-1 on lysates of HeLa, MOLT-4, and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-Cdk1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for Cdk1 protein at approximately 32 kDa.

General references:

Liu ZG, Zhao LN, Liu YW, Li TT, Fan DM, Chen JJ: Activation of Cdc2 contributes to apoptosis in HPV E6 expressing human keratinocytes in response to therapeutic agents. J Mol Biol. 2007 Nov 23;374(2):334-45.
PubMed

Lindqvist A, van Zon W, Karlsson Rosenthal C, Wolthuis RM: Cyclin B1-Cdk1 activation continues after entrosome separation to control mitotic progression. PLoS Biol. 2007 May;5(5):e123.
PubMed

Hochegger H, Dejsuphong D, Sonoda E, Saberi A, Rajendra E, Kirk J, Hunt T, Takeda S: An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells. J Cell Biol. 2007 Jul 16;178(2):257-68.
PubMed

Product specific references:

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed

Lukas J, Draetta G, Bartek J: Distinct forms of human CDC2 identified by novel monoclonal antibodies. Eur J Biochem. 1992 Jul 1;207(1):169-76.
PubMed