Exbio — Research products — Antibodies — CD and related antigens — Anti-CD49f Purified

Anti-CD49f Purified

Regulatory status

RUO

Antigen

CD49f

Clone

GoH3

Format

Purified

Reactivity

Non-human primates, Horse, Pig, Rabbit, Cow, Dog, Cat, Human, Sheep, Mouse

Application

IP, FC (QC tested), IHC-F, ICC

Variant

0.1 mg

11-913-C100

In stock

158.40 USD

Variant

0.1 mg

11-913-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a

Specificity

The rat monoclonal antibody GoH3 recognizes an extracellular epitope of CD49f, an alpha6 integrin subunit.

Workshop

HCDM workshop listed

Application

IP, FC (QC tested), IHC-F, ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Non-human primates, Horse, Pig, Rabbit, Cow, Dog, Cat, Human, Sheep, Mouse

Immunogen

mouse mammary tumor cells

Concentration

1 mg/ml

Preparation

Purified by protein-G affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ITGA6, VLA6alpha, platelet gpIc, integrin subunit alpha 6

Antigen description

CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes.

Entrez Gene ID 3655

UniProt ID P23229

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49f (GoH3) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Separation of human blood thrombocytes stained using anti-CD49f (GoH3) purified antibody (concentration in sample 1,7 μg/ml, GAM APC, red-filled) from blood thromobocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Martignani E, Ala U, Sheehy PA, Thomson PC, Baratta M: Whole transcriptome analysis of bovine mammary progenitor cells by P-Cadherin enrichment as a marker in the mammary cell hierarchy. Comparative Study Sci Rep. 2020 Aug 25;10(1):14183.
PubMed

Knapp DJ, Hammond CA, Aghaeepour N, Miller PH, Pellacani D, Beer PA, Sachs K, Qiao W, Wang W, Humphries RK, Sauvageau G, Zandstra PW, Bendall SC, Nolan GP, Hansen C, Eaves CJ: Distinct signaling programs control human hematopoietic stem cell survival and proliferation. Blood 2017 Jan 19;129(3):307-318
PubMed

Karthaus WR, Iaquinta PJ, Drost J, Gracanin A, van Boxtel R, Wongvipat J, Dowling CM, Gao D, Begthel H, Sachs N, Vries RGJ, Cuppen E, Chen Y, Sawyers CL, Clevers HC: Identification of multipotent luminal progenitor cells in human prostate organoid cultures. Cell 2014 Sep 25;159(1):163-175.
PubMed

Ikeda A, Yamamoto T, Mineshiba J, Takashiba S: Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep. 2020 Nov 17;10(1):19959.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed

Georas SN, McIntyre BW, Ebisawa M, Bednarczyk JL, Sterbinsky SA, Schleimer RP, Bochner BS: Expression of a functional laminin receptor (alpha 6 beta 1, very late activation antigen-6) on human eosinophils. Blood. 1993 Nov 1;82(9):2872-9.
PubMed