Anti-CD49f PE

Anti-CD49f PE
Regulatory status
RUO
Antigen
CD49f
Clone
GoH3
Format
PE
Reactivity
Non-human primates, Horse, Pig, Rabbit, Cow, Dog, Cat, Human, Sheep, Mouse
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-913-T100
In stock
242.00 USD
Variant
100 tests
1P-913-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Rat IgG2a
Specificity
The rat monoclonal antibody GoH3 recognizes an extracellular epitope of CD49f, an alpha6 integrin subunit.
Workshop
HCDM workshop listed
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Non-human primates, Horse, Pig, Rabbit, Cow, Dog, Cat, Human, Sheep, Mouse
Immunogen
mouse mammary tumor cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ITGA6, VLA6alpha, platelet gpIc, integrin subunit alpha 6
Antigen description
CD49f (alpha 6 integrin) is a type I transmembrane glycoprotein, which noncovalently associates with CD29 (beta 1 integrin) to form VLA-6, and with CD104 (beta 4 integrin) to form alpha6/beta4 integrin complex. CD49f is expressed on platelets, megakaryocytes, monocytes, T cells and thymocytes. It is widely expressed on many cultured adherent cell lines and on epithelia in non-lymphoid tissues. It is important for adhesion to laminins, invasin, and merosin, hence also for cell migration, embryogenesis, metastasis, formation of hemidesmosomes in epithelia, and other processes.
Entrez Gene ID 3655
UniProt ID P23229
1P-913_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD49f (GoH3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-913_FC_Histogram
Separation of human CD49f positive CD45 negative platelets (red-filled) from CD49f negative neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD49f (GoH3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Martignani E, Ala U, Sheehy PA, Thomson PC, Baratta M: Whole transcriptome analysis of bovine mammary progenitor cells by P-Cadherin enrichment as a marker in the mammary cell hierarchy. Comparative Study Sci Rep. 2020 Aug 25;10(1):14183.
PubMed
Knapp DJ, Hammond CA, Aghaeepour N, Miller PH, Pellacani D, Beer PA, Sachs K, Qiao W, Wang W, Humphries RK, Sauvageau G, Zandstra PW, Bendall SC, Nolan GP, Hansen C, Eaves CJ: Distinct signaling programs control human hematopoietic stem cell survival and proliferation. Blood 2017 Jan 19;129(3):307-318
PubMed
Karthaus WR, Iaquinta PJ, Drost J, Gracanin A, van Boxtel R, Wongvipat J, Dowling CM, Gao D, Begthel H, Sachs N, Vries RGJ, Cuppen E, Chen Y, Sawyers CL, Clevers HC: Identification of multipotent luminal progenitor cells in human prostate organoid cultures. Cell 2014 Sep 25;159(1):163-175.
PubMed
Ikeda A, Yamamoto T, Mineshiba J, Takashiba S: Follistatin expressed in mechanically-damaged salivary glands of male mice induces proliferation of CD49f + cells. Sci Rep. 2020 Nov 17;10(1):19959.
PubMed
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed
Variant
100 tests
1P-913-T100
In stock
242.00 USD
Variant
100 tests
1P-913-T100
In stock
242.00 USD

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