Exbio — Research products — Antibodies — CD and related antigens — Anti-CD370 Purified

Anti-CD370 Purified

Anti-CD370 Purified
Regulatory status
RUO
Antigen
CD370
Clone
8F9
Format
Purified
Reactivity
Human
Application
FC (QC tested), ICC
Variant
0.1 mg
11-787-C100
In stock
170.00 USD
0.025 mg
11-787-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-261-C100
In stock
170.00 USD
0.1 mg
11-261-C100
In stock
85.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.
Workshop
HLDA X
Application
FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
RBL-2H3 cells expressing human CLEC9A fused to an HA epitope
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CLEC9A, DNGR1, DNGR-1, UNQ9341
Antigen description
CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.
Entrez Gene ID 283420
UniProt ID Q6UXN8
11-787_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD370 (8F9) purified antibody (concentration in sample 1,67 µg/ml) GAM FITC.
11-787_FC_Dot-plot
Flow cytometry multicolor surface staining of human myeloid cells stained using anti-human CD370 (8F9) purified antibody (concentration in sample 1,67 µg/ml, GAM FITC) and anti-human CD16 (3G8) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
11-787_FC_Histogram
Separation of human CD370 positive CD16 positive dendritic cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD370 (8F9) purified antibody (concentration in sample 1,67 µg/ml) GAM FITC.

Product specific references:

Sancho D, Mourão-Sá D, Joffre OP, Schulz O, Rogers NC, Pennington DJ, Carlyle JR, Reis e Sousa C: Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008 Jun;118(6):2098-110. doi: 10.1172/JCI34584.
PubMed
Poulin LF, Reyal Y, Uronen-Hansson H, Schraml BU, Sancho D, Murphy KM, Håkansson UK, Moita LF, Agace WW, Bonnet D, Reis e Sousa C: DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues. Blood. 2012 Jun 21;119(25):6052-62.
PubMed
Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S: Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med. 2012 Apr 9;209(4):653-60.
PubMed
Variant
0.1 mg
11-787-C100
In stock
170.00 USD
0.025 mg
11-787-C025
Delivery 1 week
85.00 USD
Variant
0.1 mg
11-261-C100
In stock
170.00 USD
0.1 mg
11-261-C100
In stock
85.00 USD
Contact distributor
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Bulk requests
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