Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD370 PE

Anti-Hu CD370 PE

Regulatory status

RUO

Antigen

CD370

Clone

8F9

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-787-T100

Ask for delivery term HERE

242.00 USD

25 tests

1P-787-T025

Delivery 1 week

121.00 USD

Variant

100 tests

1P-787-T100

Ask for delivery term HERE

242.00 USD

25 tests

1P-787-T025

Delivery 1 week

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.

Workshop

HLDA X

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Reactivity

Human

Immunogen

RBL-2H3 cells expressing human CLEC9A fused to an HA epitope

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CLEC9A, DNGR1, DNGR-1, UNQ9341

Antigen description

CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.

Entrez Gene ID 283420

UniProt ID Q6UXN8

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD370 (8F9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human peripheral blood mononuclear cells stained using anti-human CD370 (8F9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human HLA-DR (MEM-12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD370 positive HLA-DR positive cells (red-filled) from CD370 negative HLA-DR negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD370 (8F9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Sancho D, Mourão-Sá D, Joffre OP, Schulz O, Rogers NC, Pennington DJ, Carlyle JR, Reis e Sousa C: Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008 Jun;118(6):2098-110. doi: 10.1172/JCI34584.
PubMed

Poulin LF, Reyal Y, Uronen-Hansson H, Schraml BU, Sancho D, Murphy KM, Håkansson UK, Moita LF, Agace WW, Bonnet D, Reis e Sousa C: DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues. Blood. 2012 Jun 21;119(25):6052-62.
PubMed

Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S: Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med. 2012 Apr 9;209(4):653-60.
PubMed