Anti-CD370 PE

Anti-CD370 PE
Regulatory status
RUO
Antigen
CD370
Clone
8F9
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
100 tests
1P-787-T100
In stock
220.00 USD

25 tests
1P-787-T025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD
Product details
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
RBL-2H3 cells expressing human CLEC9A fused to an HA epitope
Other names
CLEC9A, DNGR1, DNGR-1, UNQ9341
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1P-787_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD370 (8F9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-787_FC_Dot-plot
Flow cytometry multicolor surface staining of human peripheral blood mononuclear cells stained using anti-human CD370 (8F9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human HLA-DR (MEM-12) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-787_FC_Histogram
Separation of human CD370 positive HLA-DR positive cells (red-filled) from CD370 negative HLA-DR negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD370 (8F9) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Sancho D, Mourão-Sá D, Joffre OP, Schulz O, Rogers NC, Pennington DJ, Carlyle JR, Reis e Sousa C: Tumor therapy in mice via antigen targeting to a novel, DC-restricted C-type lectin. J Clin Invest. 2008 Jun;118(6):2098-110. doi: 10.1172/JCI34584.
PubMed
Poulin LF, Reyal Y, Uronen-Hansson H, Schraml BU, Sancho D, Murphy KM, Håkansson UK, Moita LF, Agace WW, Bonnet D, Reis e Sousa C: DNGR-1 is a specific and universal marker of mouse and human Batf3-dependent dendritic cells in lymphoid and nonlymphoid tissues. Blood. 2012 Jun 21;119(25):6052-62.
PubMed
Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S: Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med. 2012 Apr 9;209(4):653-60.
PubMed
Variant
100 tests
1P-787-T100
In stock
220.00 USD

25 tests
1P-787-T025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

0.1 mg
11-261-C100
In stock
110.00 USD