Anti-CD3 zeta Purified Azide Free

Anti-CD3 zeta Purified Azide Free
Regulatory status
RUO
Antigen
CD3 zeta
Clone
H146-968
Format
Purified Azide Free
Reactivity
Mouse, Human
Variant
0.1 mg
10-568-C100
In stock
120.00 USD

0.025 mg
10-568-C025
Delivery 1 week
60.00 USD
Variant
0.1 mg
11-261-C100
In stock
120.00 USD

0.1 mg
11-261-C100
In stock
60.00 USD
Product details
Description
Images
References
Isotype
Armenian Hamster IgG2 kappa
Specificity
The Armenian hamster antibody H146-968 reacts with an intracellular epitope (amino acids 151-164) of CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.
Application details
Immunocytochemistry: Recommended dilution: 4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml; reducing conditions; positive control: MOLT-4 cells, murine splenocytes.
Flow cytometry: Recommended dilution: 1-5 µg/ml. Intracellular staining.
Reactivity
Mouse, Human
Immunogen
Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CD3 zeta chain, CD247, T3Z, TCRz, IMD25
Antigen description
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Entrez Gene ID 919
UniProt ID P20963
10-568_WB
Western blotting analysis of human CD3ζ using mouse monoclonal antibody H146-968 on lysates of MOLT-4 cell line and murine splenocytes under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of armenian hamster anti-CD3ζ monoclonal antibody H146-968 followed by Alexa Fluor® 790-conjugated goat-anti-armenian hamster secondary antibody. A specific band was detected for CD3ζ protein at approximately 17 kDa.
10-568_WB_2
Western blotting analysis of CD3 zeta (CD247) in lysate of murine splenocytes (reducing conditions) by the monoclonal antibody H146-968.

General references:

Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7(5):490-5.
PubMed
Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24(2):133-9.
PubMed
Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279(28):28827-30.
PubMed

Product specific references:

Scumpia PO, Delano MJ, Kelly-Scumpia KM, Weinstein JS, Wynn JL, Winfield RD, Xia C, Chung CS, Ayala A, Atkinson MA, Reeves WH, Clare-Salzler MJ, Moldawer LL: Treatment with GITR agonistic antibody corrects adaptive immune dysfunction in sepsis. Blood. 2007 Nov 15;110(10):3673-81.
PubMed
Doyon L, Hanna Z, Jolicoeur P, Sékaly RP: Early expression of human CD4 delays thymic differentiation in transgenic mice. Res Immunol. 1994 Jan;145(1):5-16.
PubMed
Kikuchi-Maki A, Catina TL, Campbell KS: Cutting edge: KIR2DL4 transduces signals into human NK cells through association with the Fc receptor gamma protein. J Immunol. 2005 Apr 1;174(7):3859-63.
PubMed
Iida T, Ohno H, Nakaseko C, Sakuma M, Takeda-Ezaki M, Arase H, Kominami E, Fujisawa T, Saito T: Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells. J Immunol. 2000 Nov 1;165(9):5062-8.
PubMed
Kosugi A, Saitoh S, Noda S, Yasuda K, Hayashi F, Ogata M, Hamaoka T: Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation. Int Immunol. 1999 Sep;11(9):1395-401.
PubMed
Bäckström BT, Hausmann BT, Palmer E: Signaling efficiency of the T cell receptor controlled by a single amino acid in the beta chain constant region. J Exp Med. 1997 Dec 1;186(11):1933-8.
PubMed
Kishimoto H, Kubo RT, Yorifuji H, Nakayama T, Asano Y, Tada T: Physical dissociation of the TCR-CD3 complex accompanies receptor ligation. J Exp Med. 1995 Dec 1;182(6):1997-2006.
PubMed
Rozdzial MM, Kubo RT, Turner SL, Finkel TH: Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes. J Immunol. 1994 Aug 15;153(4):1563-80.
PubMed
Variant
0.1 mg
10-568-C100
In stock
120.00 USD

0.025 mg
10-568-C025
Delivery 1 week
60.00 USD
Variant
0.1 mg
11-261-C100
In stock
120.00 USD

0.1 mg
11-261-C100
In stock
60.00 USD