Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu CD235a Biotin

Anti-Hu CD235a Biotin

Regulatory status

RUO

Antigen

CD235a

Clone

JC159

Format

Biotin

Reactivity

Rat, Human

Application

FC (QC tested), IHC(F), IHC(P)

Variant

0.1 mg

1B-784-C100

In stock

198.00 USD

Variant

0.1 mg

1B-784-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

Application

FC (QC tested), IHC(F), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Rat, Human

Immunogen

Membrane preparation from splenic hairy cell leukemia

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group

Antigen description

CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.

Entrez Gene ID 2993

UniProt ID P02724

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin APC).

Separation of human CD235a positive erythrocytes (red-filled) from leukocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD235a (JC159) Biotin antibody (concentration in sample 5 μg/ml, Streptavidin APC).

General references:

Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: erythroid and megakaryocytic markers. Leuk Lymphoma. 1994;13: 401.
PubMed

Yajima A, Urano-Tashiro Y, Shimazu K, Takashima E, Takahashi Y, Konishi K: Hsa, an adhesin of Streptococcus gordonii DL1, binds to alpha2-3-linked sialic acid on glycophorin A of the erythrocyte membrane. Microbiol Immunol. 2008;52(2):69-77.
PubMed

Rogers CE, Bradley MS, Palsson BO et al: Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship withburst-forming units-erythroid. Exp Hematol. 1996; 24: 597.
PubMed

Bain BJ: Leukemia Diagnosis: a guide to the FAB classification. Gower Medical Publishing; 1990.

Keren DF, Hanson CA and Hurtubise PE, eds.: Flow Cytometry and Clinical Diagnosis. Chicago, IL: ASCP Press; 1994.

Product specific references:

Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell-derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb;99(2):441-9.
PubMed

Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb;97(2):179-83.
PubMed

Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte-bound platelets bind infectious HIV-1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25;8(11):e81002.
PubMed

Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD-based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21;121(8):1316-25.
PubMed