Exbio — Research products — Antibodies — CD and related antigens — Anti-Bov CD9 Purified

Anti-Bov CD9 Purified

Regulatory status

RUO

Antigen

CD9

Clone

IVA50

Format

Purified

Reactivity

Human, Cow

Application

FC, IP, WB

Variant

0.1 mg

11-354-C100

In stock

143.00 USD

Variant

0.1 mg

11-354-C100

In stock

143.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2

Specificity

The antibody IVA50 reacts with an extracellular epitope of CD9 antigen (bovine), a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. This antibody crossreacts with CD9 on human platelets.

Application

FC, IP, WB

Application details

Western blotting: Recommended dilution: 1 μg/ml.

Reactivity

Human, Cow

Immunogen

Bovine thrombocytes.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MIC3, MRP-1, BTCC-1, DRAP-27, TSPAN29

Antigen description

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Entrez Gene ID 280746

UniProt ID P30932

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) purified antibody (concentration in sample 10 μg/ml) GAM APC.

Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) purified antibody (concentration in sample 10 μg/ml) GAM APC.

Product specific references:

Sopp P, Howard CJ: Investigation of PC36 (BoCD45R). Vet Immunol Immunopathol. 1996 Aug;52(4):271-4.
PubMed

Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996)
PubMed

Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed

Tomaskova J, Dusinsky R, Horovska L, Simon M: A set of monoclonal antibodies specific for bovine cell surface molecule CD9. Folia Biol (Praha). 1999;45(5):225-6.
PubMed

Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L, Llanes D: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug;52(4):363-70.
PubMed