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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Bov CD62L Purified
Anti-Bov CD62L Purified
Regulatory status
RUO
Antigen
CD62L
Clone
IVA94
Format
Purified
Reactivity
Cow, Human
Application
FC, IP, ICC
Variant
0.1 mg
11-358-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody IVA94 reacts with an extracellular epitope of CD62L antigen (bovine). CD62L (L-selectin) is a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
Application
FC, IP, ICC
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Cow, Human
Immunogen
Bovine leukocytes.
Other names
L-selectin, LSEL, SELL, LAM1, LECAM1, LEU8, LNHR, LYAM1, PLNHR
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD62L (IVA94); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).
Separation of bovine CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) purified antibody (concentration in sample 1 μg/ml) GAM APC.
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) purified antibody (concentration in sample 1 μg/ml) GAM APC.
Product specific references:
Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed
Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed
Naessens J, Nthale JM, Muiya P: Biochemical analysis of preliminary clusters in the non-lineage panel. Vet Immunol Immunopathol. 1996 Aug;52(4):347-56.
PubMed
Howard CJ: Ruminant cluster CD62L. Vet Immunol Immunopathol. 1996 Aug;52(4):255-6.
PubMed
Variant
0.1 mg
11-358-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Contact distributor
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