Exbio — Research products — Antibodies — CD and related antigens — Anti-Bov CD62L Purified

Anti-Bov CD62L Purified

Regulatory status

RUO

Antigen

CD62L

Clone

IVA94

Format

Purified

Reactivity

Cow, Human

Application

FC, IP, ICC

Variant

0.1 mg

11-358-C100

In stock

143.00 USD

Variant

0.1 mg

11-358-C100

In stock

143.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody IVA94 reacts with an extracellular epitope of CD62L antigen (bovine). CD62L (L-selectin) is a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.

Application

FC, IP, ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Cow, Human

Immunogen

Bovine leukocytes.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

L-selectin, LSEL, SELL, LAM1, LECAM1, LEU8, LNHR, LYAM1, PLNHR

Antigen description

CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).

Entrez Gene ID 281485

UniProt ID P98131

Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD62L (IVA94); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).

Separation of bovine CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) purified antibody (concentration in sample 1 μg/ml) GAM APC.

Product specific references:

Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed

Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed

Naessens J, Nthale JM, Muiya P: Biochemical analysis of preliminary clusters in the non-lineage panel. Vet Immunol Immunopathol. 1996 Aug;52(4):347-56.
PubMed

Howard CJ: Ruminant cluster CD62L. Vet Immunol Immunopathol. 1996 Aug;52(4):255-6.
PubMed