Anti-Bov CD62L Alexa Fluor® 488

Anti-Bov CD62L Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
CD62L
Clone
IVA94
Format
Alexa Fluor® 488
Reactivity
Cow, Human
Excitation laser
blue (488 nm)
Variant
0.1 mg
A4-358-C100
In stock
242.00 USD
Variant
0.1 mg
A4-358-C100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody IVA94 reacts with an extracellular epitope of CD62L antigen (bovine). CD62L (L-selectin) is a 74-95 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
Application details
Flow cytometry: Recommended dilution: 1-5 µg/ml
Reactivity
Cow, Human
Immunogen
Bovine leukocytes.
Concentration
0.5 mg/ml
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
L-selectin, LSEL, SELL, LAM1, LECAM1, LEU8, LNHR, LYAM1, PLNHR
Antigen description
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).
Entrez Gene ID 281485
UniProt ID P98131
A4-358_FC_Profil Značení_2
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) Alexa Fluor® 488 antibody (concentration in sample 1 μg/ml).
A4-358_FC_Histogram
Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-CD62L (IVA94) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).
A4-358_FC_Histogram_2
Separation of bovine CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD62L (IVA94) Alexa Fluor® 488 antibody (concentration in sample 1 μg/ml).
A4-358_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-CD62L (IVA94) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).

Product specific references:

Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed
Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed
Naessens J, Nthale JM, Muiya P: Biochemical analysis of preliminary clusters in the non-lineage panel. Vet Immunol Immunopathol. 1996 Aug;52(4):347-56.
PubMed
Howard CJ: Ruminant cluster CD62L. Vet Immunol Immunopathol. 1996 Aug;52(4):255-6.
PubMed
Variant
0.1 mg
A4-358-C100
In stock
242.00 USD
Variant
0.1 mg
A4-358-C100
In stock
242.00 USD

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