Exbio — Research products — Antibodies — CD and related antigens — Anti-Bov CD41/CD61 Purified

Anti-Bov CD41/CD61 Purified

Regulatory status

RUO

Antigen

CD41/CD61

Clone

IVA30

Format

Purified

Reactivity

Cow, Human

Application

ELISA, FC, IP, ICC

Variant

0.1 mg

11-356-C100

In stock

147.40 USD

Variant

0.1 mg

11-356-C100

In stock

147.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2

Specificity

The antibody IVA30 reacts with an extracellular epitope on CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes; the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). This antibody crossreacts with human platelets.

Application

ELISA, FC, IP, ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Cow, Human

Immunogen

Bovine trombocytes.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

platelet GPIIb, Integrin alpha-IIb, GPalpha IIb, GPIIb, GT, GP3A, platelet GPIIIa

Antigen description

CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.

Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (thrombocyte-enriched suspension) using anti-bovine CD41/CD61 (IVA30); secondary antibody conjugated to FITC (green signal at thrombocytes), DNA stained with DAPI (blue signal).

Separation of human CD41/CD61 positive blood debris (red-filled) from CD41/CD61 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).

Separation of bovine CD41/CD61 positive thrombocytes (red-filled) from CD41/CD61 negative leukocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Product specific references:

Mateo A, Pintado CO, Perez de la Lastra J, Dusinsky R, Simon M, Naessens J, Llanes D: Ruminant cluster CD41/CD61. Vet Immunol Immunopathol. 1996 Aug;52(4):251-3.
PubMed

Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed

Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L, Llanes D: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug;52(4):363-70.
PubMed

Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed

Simon M, Dusinsky R, Horovska L, Bilka F, Hluchy S: Immunohistochemical reactivity of anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug;52(4):377-82.
PubMed