Anti-Bov CD41/CD61 Purified

Anti-Bov CD41/CD61 Purified
Regulatory status
RUO
Antigen
CD41/CD61
Clone
IVA30
Format
Purified
Reactivity
Cow, Human
Variant
0.1 mg
11-356-C100
In stock
143.00 USD
Variant
0.1 mg
11-356-C100
In stock
143.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2
Specificity
The antibody IVA30 reacts with an extracellular epitope on CD41/CD61 complex (bovine), one of the earliest markers of the megakaryocytic lineage. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, vitronectin, thrombin and mediates platelets aggregation. CD41/CD61 is expressed only by platelets and megakaryocytes; the complex may be absent or strongly reduced in Glanzmann's thrombasthenia (GT). This antibody crossreacts with human platelets.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Cow, Human
Immunogen
Bovine trombocytes.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
platelet GPIIb, Integrin alpha-IIb, GPalpha IIb, GPIIb, GT, GP3A, platelet GPIIIa
Antigen description
CD41 (platelet glycoprotein IIb, integrin alpha IIb) is composed of two subunits (120 kDa transmembrane alpha chain and 23 kDa extracellular beta chain) and interacts with CD61 (platelet glycoprotein IIIa, integrin beta 3) in the presence of calcium to form a functional adhesive protein receptor. CD41/CD61 complex is one of the earliest markers of the megakaryocytic lineage. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin, and it is involved in platelet aggregation.
11-356_ICC
Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (thrombocyte-enriched suspension) using anti-bovine CD41/CD61 (IVA30); secondary antibody conjugated to FITC (green signal at thrombocytes), DNA stained with DAPI (blue signal).
11-356_FC_Histogram
Separation of human CD41/CD61 positive blood debris (red-filled) from CD41/CD61 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).
11-356_FC_Histogram_2
Separation of bovine CD41/CD61 positive thrombocytes (red-filled) from CD41/CD61 negative leukocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 3 μg/ml, GAM APC).
11-356_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).
11-356_FC_Profil Značení_2
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD41/CD61 (IVA30) purified antibody (concentration in sample 3 μg/ml, GAM APC).

Product specific references:

Mateo A, Pintado CO, Perez de la Lastra J, Dusinsky R, Simon M, Naessens J, Llanes D: Ruminant cluster CD41/CD61. Vet Immunol Immunopathol. 1996 Aug;52(4):251-3.
PubMed
Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed
Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L, Llanes D: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug;52(4):363-70.
PubMed
Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed
Simon M, Dusinsky R, Horovska L, Bilka F, Hluchy S: Immunohistochemical reactivity of anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug;52(4):377-82.
PubMed
Variant
0.1 mg
11-356-C100
In stock
143.00 USD
Variant
0.1 mg
11-356-C100
In stock
143.00 USD

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