Exbio — Research products — Antibodies — CD and related antigens — Anti-Bov CD18 Purified

Anti-Bov CD18 Purified

Regulatory status

RUO

Antigen

CD18

Clone

IVA35

Format

Purified

Reactivity

Cow, Human

Application

FC, IP, ICC

Variant

0.1 mg

11-355-C100

In stock

143.00 USD

Variant

0.1 mg

11-355-C100

In stock

143.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody IVA35 reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.

Application

FC, IP, ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Cow, Human

Immunogen

Bovine leukocytes.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Integrin beta2, Complement receptor C3 subunit beta, ITGB2

Antigen description

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Entrez Gene ID 281877

UniProt ID P32592

Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD18 (IVA35); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).

Separation of bovine lymphocytes stained using anti-bovine CD18 (IVA35) purified antibody (concentration in sample 10 μg/ml, GAM APC, red-filled) from bovine lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD18 (IVA35) purified antibody (concentration in sample 10 μg/ml) GAM APC.

Product specific references:

Naessens J & Hopkins J Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed

Dusinsky R, Tomaskova J, Horovska L, Simon M: Monoclonal antibodies specific for bovine CD18. Folia Biol (Praha). 2001;47(3):108-10.
PubMed

Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed