Anti-Bov CD18 Purified

Anti-Bov CD18 Purified
Regulatory status
RUO
Antigen
CD18
Clone
IVA35
Format
Purified
Reactivity
Cow, Human
Application
Variant
0.1 mg
11-355-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody IVA35 reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
Application
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Cow, Human
Immunogen
Bovine leukocytes.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Integrin beta2, Complement receptor C3 subunit beta, ITGB2
Antigen description
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Entrez Gene ID 281877
UniProt ID P32592
11-355_ICC
Immunocytochemistry staining of acetone/methanol fixed bovine peripheral blood (lymphocyte-enriched suspension) using anti-bovine CD18 (IVA35); secondary antibody conjugated to FITC (green), DNA stained with DAPI (blue).
11-355_FC_Histogram
Separation of bovine lymphocytes stained using anti-bovine CD18 (IVA35) purified antibody (concentration in sample 10 μg/ml, GAM APC, red-filled) from bovine lymphocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).
11-355_FC_Profil Značení
Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD18 (IVA35) purified antibody (concentration in sample 10 μg/ml) GAM APC.

Product specific references:

Naessens J & Hopkins J Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed
Dusinsky R, Tomaskova J, Horovska L, Simon M: Monoclonal antibodies specific for bovine CD18. Folia Biol (Praha). 2001;47(3):108-10.
PubMed
Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed
Variant
0.1 mg
11-355-C100
In stock
130.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD