Exbio — Research products — Antibodies — CD and related antigens — Anti-Bov CD18 Alexa Fluor<sup>®</sup> 488

Anti-Bov CD18 Alexa Fluor^® 488

Regulatory status

RUO

Antigen

CD18

Clone

IVA35

Format

Alexa Fluor^® 488

Reactivity

Cow, Human

Application

FC (QC tested), ICC

Excitation laser

blue (488 nm)

Variant

0.1 mg

A4-355-C100

In stock

242.00 USD

Variant

0.1 mg

A4-355-C100

In stock

242.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody IVA35 reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.

Application

FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 2-5 µg/ml

Reactivity

Cow, Human

Immunogen

Bovine leukocytes.

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Integrin beta2, Complement receptor C3 subunit beta, ITGB2

Antigen description

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Entrez Gene ID 281877

UniProt ID P32592

Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD18 (IVA35) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml).

Separation of human neutrophil granulocytes (red-filled) from human CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-CD18 (IVA35) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).

Separation of bovine leukocytes stained using anti-bovine CD18 (IVA35) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml, red-filled) from bovine leukocytes stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 488 antibody (concentration in sample 3 μg/ml, black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-CD18 (IVA35) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).

Product specific references:

Naessens J & Hopkins J Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996).
PubMed

Dusinsky R, Tomaskova J, Horovska L, Simon M: Monoclonal antibodies specific for bovine CD18. Folia Biol (Praha). 2001;47(3):108-10.
PubMed

Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8.
PubMed