Exbio — Research products — Antibodies — CD and related antigens — Anti-A2aR Purified

Anti-A2aR Purified

Regulatory status

RUO

Antigen

A2aR

Clone

7F6-G5-A2

Format

Purified

Reactivity

Rabbit, Dog, Mouse, Rat, Human, Sheep, Guinea pig

Application

ICC (reported), IHC-P (reported), IHC-F (reported), FC-IC (QC tested), WB (reported)

test1 True

Variant

0.1 mg

11-951-C100

In stock

203.50 USD

Variant

0.1 mg

11-951-C100

In stock

203.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

The mouse monoclonal antibody 7F6-G5-A2 recognizes the epitope SQPLPGER within the third intracellular loop of A2aR, a 43 kDa transmembrane protein of the G protein-coupled receptor family.

Application

ICC (reported), IHC-P (reported), IHC-F (reported), FC-IC (QC tested), WB (reported)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Reactivity

Rabbit, Dog, Mouse, Rat, Human, Sheep, Guinea pig

Immunogen

recombinant full length human A2aR

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Adenosine A2a receptor, ADORA2A, AA2AR, RDC8

Antigen description

Adenosine A2a receptor (A2aR) is expressed mainly in the brain, heart and on cells of the immune system. Its ligand, extracellular adenonosine, is generated in local tissues under hypoxic, ischemic or inflammatory conditions, and can lead to increased A2aR expression. This receptor has neuromodulatory functions, affects cardiac rhythm, cerebral and renal blood flow, sleep, neurodegenerative disorders, and is involved also in pain regulation. It is a novel therapeutic target for depression. In human and murine monocytes and macrophages A2aR modulates the production of inflammatory cytokines.

Entrez Gene ID 135

UniProt ID P29274

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-A2aR (7F6-G5-A2) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Separation of human monocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using using anti-A2aR (7F6-G5-A2) purified antibody (concentration in sample 5 μg/ml, GAM APC).

Product specific references:

Koshiba M, Rosin DL, Hayashi N, Linden J, Sitkovsky MV: Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies. Mol Pharmacol. 1999 Mar;55(3):614-24.
PubMed

Khoa ND, Montesinos MC, Reiss AB, Delano D, Awadallah N, Cronstein BN: Inflammatory cytokines regulate function and expression of adenosine A(2A) receptors in human monocytic THP-1 cells. J Immunol. 2001 Oct 1;167(7):4026-32.
PubMed

Oliveira L, Correia A, Cristina Costa A, Guerra-Gomes S, Ferreirinha F, Magalhães-Cardoso MT, Vilanova M, Correia-de-Sá P: Deficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis. Mediators Inflamm. 2015:2015:460610.
PubMed

Rossetto IM, Cagnon VH, Kido LA, Lizarte FS, Tirapelli LF, Tirapelli DP, de Almeida LG, Martinez FE, Martinez M: Caffeine consumption attenuates ethanol-induced inflammation through the regulation of adenosinergic receptors in the UChB rats cerebellum. Toxicol Res (Camb). 2021 Jul 24;10(4):835-849.
PubMed

Godoy-Marín H, Jiménez-Sábado V, Tarifa C, Ginel A, Santos JL, Bentzen BH, Hove-Madsen L, Ciruela F: Increased density of endogenous adenosine A2A receptors in atrial fibrillation: From cellular and porcine models to human patients. Int J Mol Sci. 2023 Feb 11;24(4):3668.
PubMed

Wang M, Li P, Li Z, da Silva BS, Zheng W, Xiang Z, He Y, Xu T, Cordeiro C, Deng L, Dai Y, Ye M, Lin Z, Zhou J, Zhou X, Ye F, Cunha RA, Chen J, Guo W: Lateral septum adenosine A2A receptors control stress-induced depressive-like behaviors via signaling to the hypothalamus and habenula. Nat Commun. 2023 Apr 5;14(1):1880.
PubMed