Exbio — Research products — Antibodies — Blood group antigens — Anti-Sialyl Lewis a Purified

Anti-Sialyl Lewis a Purified

Regulatory status

RUO

Antigen

Sialyl Lewis a

Clone

121SLE

Format

Purified

Reactivity

Human

Application

ELISA, IHC(P)

Variant

0.1 mg

11-285-C100

In stock

121.00 USD

0.025 mg

11-285-C025

Delivery 1 week

60.50 USD

Variant

0.1 mg

11-285-C100

In stock

121.00 USD

0.025 mg

11-285-C025

Delivery 1 week

60.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody 121SLE recognizes Sialyl Lewis a-containing glycolipids (extracellular plasma membrane leaflet antigens).

Workshop

1st international workshop on Mabs against human red blood cell and related antigens, Paris, 1987

Application

ELISA, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation 1 hour / RT; detection DAB.

Reactivity

Human

Immunogen

Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CA-19.9

Immunohistochemistry staining of sialyl-Lewis a in colorectal adenocarcinoma (paraffin-embedded sections) with 121SLE antibody.

Immunohistochemistry staining of human pancreas (paraffin-embedded sections) with anti-sialyl Lewis a (121SLE), 10 μg/ml. Commercially tested by LifeSpan BioSciences.

Product specific references:

Rouger Ph. et al. (Eds.): Proceedings of the First International Workshop on monoclonal antibodies against human red blood cells and related antigens (Paris, 1987) published in: Blood Transfusion and Immunohaematology 30 (5), pp 353-720.

Rye PD, Bovin NV, Vlasova EV, Molodyk AA, Baryshnikov A, Kreutz FT, Garinther WI, Schultes BC, Noujaim AA, Madiyalakan R, Magnani J, Nilsson O, Nilsson K, Nustad K, Norum L, Bell H, Cao Y, Suresh MR, Very DL, Freeman JV, Yeung KK, Hilgers J: Summary report on the ISOBM TD-6 workshop: analysis of 20 monoclonal antibodies against Sialyl Lewisa and related antigens. Montreux, Switzerland, September 19-24, 1997. Tumour Biol. 1998;19(5):390-420.
PubMed