Exbio — Research products — Antibodies — Blood group antigens — Anti-Hu CD108 Purified

Anti-Hu CD108 Purified

Regulatory status

RUO

Antigen

CD108

Clone

MEM-150

Format

Purified

Reactivity

Human

Application

IP, WB, FC (QC tested)

Variant

0.1 mg

11-275-C100

In stock

154.00 USD

0.025 mg

11-275-C025

Delivery 1 week

77.00 USD

Variant

0.1 mg

11-275-C100

In stock

154.00 USD

0.025 mg

11-275-C025

Delivery 1 week

77.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody MEM-150 reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored extracellular glycoprotein expressed on various cell types including erythrocytes, lymphoblasts; at low levels it is present on circulating lymphocytes.

Workshop

HLDA V: WS Code AS S017; HLDA V: WS Code BP BP347; HLDA VI: WS Code BP 401; HLDA VI: WS Code BP 475; HLDA VI: WS Code NL N-L156; HLDA VI: WS Code P PR-65

Application

IP, WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 4 μg/ml.
Western blotting: Non-reducing conditions.

Reactivity

Human

Immunogen

HPB-ALL human T cell line

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Semaphorin 7a, JMH blood group antigen, SEMAL, SEMAK1

Antigen description

CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors – plexin C1 and beta1 integrins.

Entrez Gene ID 8482

UniProt ID O75326

Flow cytometry analysis (surface staining) of human peripheral blood with anti-human CD108 (MEM-150) purified, GAM IgM PE.

General references:

Pasterkamp RJ, Peschon JJ, Spriggs MK, Kolodkin AL: Semaphorin 7A promotes axon outgrowth through integrins and MAPKs. Nature. 2003 Jul 24;424(6947):398-405.
PubMed

Delorme G, Saltel F, Bonnelye E, Jurdic P, Machuca-Gayet I: Expression and function of semaphorin 7A in bone cells. Biol Cell. 2005 Jul;97(7):589-97.
PubMed

Pasterkamp RJ, Kolk SM, Hellemons AJ, Kolodkin AL: Expression patterns of semaphorin7A and plexinC1 during rat neural development suggest roles in axon guidance and neuronal migration. BMC Dev Biol. 2007 Aug 29;7:98.
PubMed

Suzuki K, Okuno T, Yamamoto M, Pasterkamp RJ, Takegahara N, Takamatsu H, Kitao T, Takagi J, Rennert PD, Kolodkin AL, Kumanogoh A, Kikutani H: Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin. Nature. 2007 Apr 5;446(7136):680-4.
PubMed

Product specific references:

Angelisova P, Drbal K, Cerny J, Hilgert I, Horejsi V: Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology. 1999 Jun;200(2):234-45.
PubMed

Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).

Mudad R, Rao N, Angelisova P, Horejsi V, Telen MJ.: Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion. 1995 Jul;35(7):566-70.
PubMed