Anti-Hu CD108 FITC

Anti-Hu CD108 FITC
Regulatory status
RUO
Antigen
CD108
Clone
MEM-150
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-275-T100
In stock
180.00 USD

25 tests
1F-275-T025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
References
Isotype
Mouse IgM
Specificity
The antibody MEM-150 reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored extracellular glycoprotein expressed on various cell types including erythrocytes, lymphoblasts; at low levels it is present on circulating lymphocytes.
Workshop
HLDA V: WS Code AS S017; HLDA V: WS Code BP BP347; HLDA VI: WS Code BP 401; HLDA VI: WS Code BP 475; HLDA VI: WS Code NL N-L156; HLDA VI: WS Code P PR-65
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
HPB-ALL human T cell line
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Semaphorin 7a, JMH blood group antigen, SEMAL, SEMAK1
Antigen description
CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors – plexin C1 and beta1 integrins.
Entrez Gene ID 8482
UniProt ID O75326

General references:

Pasterkamp RJ, Peschon JJ, Spriggs MK, Kolodkin AL: Semaphorin 7A promotes axon outgrowth through integrins and MAPKs. Nature. 2003 Jul 24;424(6947):398-405.
PubMed
Delorme G, Saltel F, Bonnelye E, Jurdic P, Machuca-Gayet I: Expression and function of semaphorin 7A in bone cells. Biol Cell. 2005 Jul;97(7):589-97.
PubMed
Pasterkamp RJ, Kolk SM, Hellemons AJ, Kolodkin AL: Expression patterns of semaphorin7A and plexinC1 during rat neural development suggest roles in axon guidance and neuronal migration. BMC Dev Biol. 2007 Aug 29;7:98.
PubMed
Suzuki K, Okuno T, Yamamoto M, Pasterkamp RJ, Takegahara N, Takamatsu H, Kitao T, Takagi J, Rennert PD, Kolodkin AL, Kumanogoh A, Kikutani H: Semaphorin 7A initiates T-cell-mediated inflammatory responses through alpha1beta1 integrin. Nature. 2007 Apr 5;446(7136):680-4.
PubMed

Product specific references:

Angelisova P, Drbal K, Cerny J, Hilgert I, Horejsi V: Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules. Immunobiology. 1999 Jun;200(2):234-45.
PubMed
Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
Mudad R, Rao N, Angelisova P, Horejsi V, Telen MJ.: Evidence that CDw108 membrane protein bears the JMH blood group antigen. Transfusion. 1995 Jul;35(7):566-70.
PubMed
Variant
100 tests
1F-275-T100
In stock
180.00 USD

25 tests
1F-275-T025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD

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