Anti-Blood Group Lewis b Purified

Anti-Blood Group Lewis b Purified
Regulatory status
RUO
Antigen
Blood Group Lewis b
Clone
2-25LE
Format
Purified
Reactivity
Human
Application
ELISA, WB, IHC(P)
Variant
0.1 mg
11-386-C100
In stock

0.025 mg
11-386-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.
Application
ELISA, WB, IHC(P)
Reactivity
Human
Immunogen
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-386_IHC
Immunohistochemistry staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE). Commercially tested by LifeSpan BioSciences.
11-386_IHC_2
Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

General references:

Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254(1):185-93.
PubMed
Torrado J, Correa P, Ruiz B, Bernardi P, Závala D, Bara J: Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30.
PubMed

Product specific references:

First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems.
Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J: Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631-6.
PubMed
Variant
0.1 mg
11-386-C100
In stock

0.025 mg
11-386-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock