Exbio — Research products — Antibodies — Blood group antigens — Anti-Blood Group Lewis b Purified

Anti-Blood Group Lewis b Purified

Anti-Blood Group Lewis b Purified
Regulatory status
RUO
Antigen
Blood Group Lewis b
Clone
2-25LE
Format
Purified
Reactivity
Human
Application
ELISA, WB, IHC-P
Variant
0.1 mg
11-386-C100
In stock
124.30 USD
0.025 mg
11-386-C025
Delivery 1 week
62.15 USD
Variant
0.1 mg
11-386-C100
In stock
124.30 USD
0.025 mg
11-386-C025
Delivery 1 week
62.15 USD
Contact distributor
Product details
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody 2-25LE reacts with Lewisb blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.
Workshop
First international workshop on monoclonal antibodies against human red blood cell and related antigens, Paris, September 1987, WS code 34W2
Application
ELISA, WB, IHC-P
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation: 1 hour / RT; detection DAB.
Reactivity
Human
Immunogen
Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-386_IHC
Immunohistochemistry staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE). Commercially tested by LifeSpan BioSciences.
11-386_IHC_2
Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

General references:

Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254(1):185-93.
PubMed
Torrado J, Correa P, Ruiz B, Bernardi P, Závala D, Bara J: Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30.
PubMed

Product specific references:

First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems.
Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J: Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631-6.
PubMed
Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254(1):185-93.
PubMed
Gousset K, Schiff E, Langevin C, Marijanovic Z, Caputo A, Browman DT, Chenouard N, de Chaumont F, Martino A, Enninga J, Olivo-Marin JC, Männel D, Zurzolo C: Prions hijack tunnelling nanotubes for intercellular spread. Nat Cell Biol. 2009 Mar;11(3):328-36.
PubMed
Matza D, Kerem A, Medvedovsky H, Lantner F, Shachar I: Invariant chain-induced B cell differentiation requires intramembrane proteolytic release of the cytosolic domain. Immunity. 2002 Nov;17(5):549-60.
PubMed
Variant
0.1 mg
11-386-C100
In stock
124.30 USD
0.025 mg
11-386-C025
Delivery 1 week
62.15 USD
Variant
0.1 mg
11-386-C100
In stock
124.30 USD
0.025 mg
11-386-C025
Delivery 1 week
62.15 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product