Exbio — Research products — Antibodies — Blood group antigens — Anti-Blood Group Lewis b Purified

Anti-Blood Group Lewis b Purified

Regulatory status

RUO

Antigen

Blood Group Lewis b

Clone

2-25LE

Format

Purified

Reactivity

Human

Application

ELISA, WB, IHC(P)

Variant

0.1 mg

11-386-C100

In stock

110.00 USD

0.025 mg

11-386-C025

Delivery 1 week

55.00 USD

Variant

0.1 mg

11-386-C100

In stock

110.00 USD

0.025 mg

11-386-C025

Delivery 1 week

55.00 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody 2-25LE reacts with Lewis^b blood group antigen, a carbohydrate determinant carried on both glycolipids and glycoproteins, detected on the surface of erythrocytes, certain epithelial cells, and in secretions of certain individuals.

Workshop

First international workshop on monoclonal antibodies against human red blood cell and related antigens, Paris, September 1987, WS code 34W2

Application

ELISA, WB, IHC(P)

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colorectal adenocarcinoma; heat mediated antigen retrieval (sodium citrate); mAb incubation: 1 hour / RT; detection DAB.

Reactivity

Human

Immunogen

Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio´s classification).

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Immunohistochemistry staining of human small intestine (paraffin sections) using anti-blood group Lewis b (clone 2-25LE). Commercially tested by LifeSpan BioSciences.

Immunohistochemistry staining of human colon adenocarcinoma (paraffin sections) using anti-blood group Lewis b (clone 2-25LE).

General references:

Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254(1):185-93.
PubMed

Torrado J, Correa P, Ruiz B, Bernardi P, Závala D, Bara J: Lewis antigen alterations in gastric cancer precursors. Gastroenterology. 1992 Feb;102(2):424-30.
PubMed

Product specific references:

First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Paris, September 21-24, 1987. Vol. II: Other blood group systems.

Creuzot-Garcher C, Guerzider V, Assem M, Bron AM, Delannoy P, Bara J: Alteration of sialyl Lewis epitope expression in pterygium. Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1631-6.
PubMed

Bara J, Gautier R, Le Pendu J, Oriol R: Immunochemical characterization of mucins. Polypeptide (M1) and polysaccharide (A and Leb) antigens. Biochem J. 1988 Aug 15;254(1):185-93.
PubMed

Gousset K, Schiff E, Langevin C, Marijanovic Z, Caputo A, Browman DT, Chenouard N, de Chaumont F, Martino A, Enninga J, Olivo-Marin JC, Männel D, Zurzolo C: Prions hijack tunnelling nanotubes for intercellular spread. Nat Cell Biol. 2009 Mar;11(3):328-36.
PubMed

Matza D, Kerem A, Medvedovsky H, Lantner F, Shachar I: Invariant chain-induced B cell differentiation requires intramembrane proteolytic release of the cytosolic domain. Immunity. 2002 Nov;17(5):549-60.
PubMed