Exbio — Research products — Antibodies — Blood group antigens — Anti-Blood Group ABH Purified

Anti-Blood Group ABH Purified

Regulatory status

RUO

Antigen

Blood Group ABH

Clone

HE-10

Format

Purified

Reactivity

Human

Application

FC (QC tested), IHC(P)

Variant

0.1 mg

11-812-C100

In stock

154.00 USD

Variant

0.1 mg

11-812-C100

In stock

154.00 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALe^b. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.

Application

FC (QC tested), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry surface staining pattern of human peripheral whole blood from group A donor stained using anti-blood group ABH (HE-10) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Separation of human erythrocytes from blood group A donor (red-filled) from erythrocytes from blood group 0 donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples using anti-Blood group ABH (HE-10) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Product specific references:

Nemec M, Drimalova D, Horejsi V, Vanak J, Bartek J, Viklicky V: Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang. 1987;52(1-2):125-8.
PubMed

Vanak J, Drimalova D, Smyslova O, Nemec M, Viklicky V, Wisniewski K: Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88.
PubMed