Exbio — Research products — Antibodies — Blood group antigens — Anti-Blood Group ABH PE

Anti-Blood Group ABH PE

Regulatory status

RUO

Antigen

Blood Group ABH

Clone

HE-10

Format

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-812-C100

Ask for delivery term HERE

308.00 USD

Variant

0.1 mg

1P-812-C100

Ask for delivery term HERE

308.00 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALe^b. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2-5 μg/ml.

Reactivity

Human

Immunogen

Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Blood Group ABH (HE-10) PE antibody (concentration in sample 5 μg/ml).

Separation of erythrocytes stained using anti-human Blood group ABH (HE-10) PE antibody (concentration in sample 5 μg/ml) from erythrocytes stained using mouse IgM isotype control (PFR-03) PE antibody (concentration in sample 5 μg/ml, same as anti-human Blood group ABH PE concentration, black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood.

Product specific references:

Nemec M, Drimalova D, Horejsi V, Vanak J, Bartek J, Viklicky V: Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang. 1987;52(1-2):125-8.
PubMed

Vanak J, Drimalova D, Smyslova O, Nemec M, Viklicky V, Wisniewski K: Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88.
PubMed