Anti-Blood Group ABH Culture supernatant

Anti-Blood Group ABH Culture supernatant
Regulatory status
RUO
Antigen
Blood Group ABH
Clone
HE-10
Format
Culture supernatant
Reactivity
Human
Application
FC (QC tested), IHC(P)
Variant
1.0 ml
99-812-L001
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Images
References
Isotype
Mouse IgM
Specificity
The mouse monoclonal antibody HE-10 agglutinates erythrocytes of group A, and is excellent as a tumour marker in patients of blood group B and 0. It does not agglutinate erythrocytes of group B and 0. Study with specific oligosaccharides showed that the antibody HE-10 reacts with A and H antigens with chain types 3 and 4 and it does not react with A disaccharide, A trisaccharide, A type 1, A type 2, ALeb. The antibody HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues.
Application
FC (QC tested), IHC(P)
Application details
Immunohistochemistry (paraffin sections): The antibody HE-10 is excellent as a tumour marker in patients of blood group B and 0.
Flow cytometry: Recommended dilution: 1-3 : 100.
Reactivity
Human
Immunogen
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Preparation
The hybridoma culture supernatant is 4 x concentrated by ultrafiltration using 100 kDa-cut off membrane.
Formulation
Culture supernatant
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
99-812_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood from group A donor stained using anti-blood group ABH (HE-10) antibody (culture supernatant, GAM FITC).
99-812_FC_Histogram
Separation of human erythrocytes from blood group A donor (red-filled) from erythrocytes from blood group 0 donor (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood samples using anti-blood group ABH (HE-10) antibody (culture supernatant, GAM FITC).

Product specific references:

Nemec M, Drimalova D, Horejsi V, Vanak J, Bartek J, Viklicky V: Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang. 1987;52(1-2):125-8.
PubMed
Vanak J, Drimalova D, Smyslova O, Nemec M, Viklicky V, Wisniewski K: Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88.
PubMed
Variant
1.0 ml
99-812-L001
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
90.00 USD