Exbio — Research products — Antibodies — Blood group antigens — Anti-Blood Group A Purified

Anti-Blood Group A Purified

Regulatory status

RUO

Antigen

Blood Group A

Clone

HE-195

Format

Purified

Reactivity

Human

Application

FC (QC tested)

Variant

0.1 mg

11-805-C100

In stock

154.00 USD

Variant

0.1 mg

11-805-C100

In stock

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody HE-195 recognizes human blood group A including weak variants A₃, A_X, A₃B, A_XB. The specifity of antibody HE-195 was confirmed by comparison of specifity and reactivity to standard reagent using >5000 samples of blood.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Blood Group A (HE-195) purified antibody (concentration in sample 3,3 μg/ml, GAM APC).

Separation of erythrocytes stained anti-human Blood Group A (HE-195) purified antibody (concentration in sample 3,3 μg/ml, GAM APC, red-filled) from erythrocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Nemec M, Drimalova D, Horejsi V, Vanak J, Bartek J, Viklicky V: Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang. 1987;52(1-2):125-8.
PubMed