Anti-Blood Group A Purified

Anti-Blood Group A Purified
Regulatory status
RUO
Antigen
Blood Group A
Clone
HE-195
Format
Purified
Reactivity
Human
Application
Variant
0.1 mg
11-805-C100
In stock
154.00 USD
Variant
0.1 mg
11-805-C100
In stock
154.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgM
Specificity
The antibody HE-195 recognizes human blood group A including weak variants A3, AX, A3B, AXB. The specifity of antibody HE-195 was confirmed by comparison of specifity and reactivity to standard reagent using >5000 samples of blood.
Application
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
Mixture of erythrocytes of group A1 and glycoprotein fraction isolated from saliva of secretors with blood group A.
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
Human blood group A antigen belongs to a group of carbohydrate determinats carried on both glycolipids and glycoproteins; it is detected on erythrocytes and certain epithelial cells.
11-805_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human Blood Group A (HE-195) purified antibody (concentration in sample 3,3 μg/ml, GAM APC).
11-805_FC_Histogram
Separation of erythrocytes stained anti-human Blood Group A (HE-195) purified antibody (concentration in sample 3,3 μg/ml, GAM APC, red-filled) from erythrocytes unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Nemec M, Drimalova D, Horejsi V, Vanak J, Bartek J, Viklicky V: Murine monoclonal antibodies to human A erythrocytes: differential reactivity with N-acetyl-D-galactosamine. Vox Sang. 1987;52(1-2):125-8.
PubMed
Variant
0.1 mg
11-805-C100
In stock
154.00 USD
Variant
0.1 mg
11-805-C100
In stock
154.00 USD

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