Exbio — Research products — Accessory reagents — Streptavidin — Streptavidin PE

Streptavidin PE

Regulatory status

RUO

Format

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

EXB0042

In stock

88.00 USD

Variant

0.1 mg

EXB0042

In stock

88.00 USD

Contact distributor

Product details

Images

SDS download

Application

FC (QC tested)

Application details

Flow cytometry: Recomended use: 1 μg/ml. Extracellular and intracellular staining.

Description

Streptavidin PE is intended for detecting biotinylated antigens and biotinylated antibodies by flow cytometry.

Contents

Streptavidin PE - 1 vial (0.5 ml) of Ready-to-use solution. No reconstitution is necessary. Reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.

Preparation

Purified recombinant protein is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated protein and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD4 (MEM-241) Biotin antibody and secondary stained using Streptavidin PE (concentration in sample 1 μg/ml).

Flow cytometry multicolor surface staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human lymphocytes using anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood) and intracellular staining of human lymphocytes using anti-human IL-2 (35C3) Biotin antibody (concentration in sample 0.5 μg/ml) and secondary stained using Streptavidin PE (concentration in sample 1 μg/ml).

Flow cytometry intracellular staining pattern of PMA + Ionomycin stimulated and Brefeldin A treated human peripheral whole blood stained using anti-human IL-2 (35C3) Biotin antibody and secondary stained using Streptavidin PE (concentration in sample 1 μg/ml).