Recombinant mouse NTAL / LAB

Recombinant mouse NTAL / LAB
Regulatory status
RUO
Format
Purified
Reactivity
Mouse
Application
WB
Variant
0.1 mg
EXB0010
In stock
290.00 USD
Variant
0.1 mg
11-261-C100
In stock
290.00 USD
Product details
Images
Application
WB
Reactivity
Mouse
Description
Recombinant mouse NTAL / LAB is expressed in E. coli. DNA sequence encoding mouse NTAL (amino acids 30 - 203) was fused with a His tag at the N-terminus.
A calculated molecular mass of the fussion protein is 22.2 kDa. The recombinant fragment migrates as a 28 kDa protein in SDS-PAGE under reducing conditions.
Contents
Recombinant mouse NTAL / LAB - 1 vial (0.1 mg).
Reconstitute vial content with 100 μl sterile deionized water.
For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
Preparation
The protein was purified by immobilized metal affinity chromatography used for purification of polyhistidine-tagged recombinant proteins, and lyophilized.
Formulation
Lyophilized from a 0.22 μm filtered solution in phosphate buffered saline (PBS).
Storage and handling
Store lyophilized product in freezer (-20 °C to -80 °C). The expiration date of unopened product vial is indicated on the vial label. After reconstitution prepare suitable aliquots. Reconstituted product is stable for at least 6 months when stored in freezer (-20 °C to -80 °C). Avoid freeze/thaw cycles.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
EXB0010_WB_2
Western Blotting analysis of mouse NTAL / LAB recombinant protein (Cat. No. EXB0010, previously RL-005-C100). SDS-PAGE Electrophoresis (12% separating gel, 6% stacking gel; reducing conditions). Lane 1 - 4: immunostaining with NTAL specific antibody; dilution 2 μg/ml). Detection with Goat anti-mouse/ HRP secondary antibody. Amount of mouse NTAL / LAB recombinant protein loaded on each lane: (1) 50 ng, (2) 20 ng, (3) 5 ng, (4) 1 ng.
EXB0010_WB_1
SDS-PAGE Electrophoresis of mouse NTAL / LAB recombinant protein (Cat. No. EXB0010, previously RL-005-C100; 12% separating gel, 6% stacking gel; reducing conditions). Lane 1 - 4: mouse NTAL / LAB recombinant protein (2000 ng, 1000 ng, 500 ng, 200 ng per lane). Staining with Coomassie Blue.
Variant
0.1 mg
EXB0010
In stock
290.00 USD
Variant
0.1 mg
11-261-C100
In stock
290.00 USD

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