Exbio — Research products — Accessory reagents — Annexin V — Annexin V Dyomics 647

Annexin V Dyomics 647

Regulatory status

RUO

Format

Dyomics 647

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

EXB0023

In stock

220.00 USD

500 tests

EXB0030

Ask for delivery term HERE

737.00 USD

Variant

100 tests

EXB0023

In stock

220.00 USD

500 tests

EXB0030

Ask for delivery term HERE

737.00 USD

Contact distributor

Product details

Images

SDS download

Application

FC (QC tested)

Description

Annexin V Dyomics 647 is intended for detection of early apoptotic cells by flow cytometry.

Contents

Annexin V Dyomics 647 - 1 vial (0.5 ml / 2.5 ml) of Ready-to-use solution. No reconstitution is necessary. Reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. Sufficient for 100 / 500 tests.

Preparation

Purified recombinant protein is conjugated with Dyomics 647 under optimum conditions. Unconjugated protein and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Separation of Annexin positive Hoechst 34580 positive events (red-filled) from Annexin negative Hoechst 34580 negative events (black-dashed) in flow cytometry analysis (surface staining) of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 μl reagent per million cells in 100 μl of cell suspension).

Flow cytometry multicolor surface staining of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 μl reagent per million cells in 100 μl of cell suspension) and Hoechst 34580 viability stain.

Separation of Annexin stained apoptotic Jurkat cells (red-filled) from unstained cells (black-dashed) in flow cytometry analysis (surface staining) of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 μl reagent per million cells in 100 μl of cell suspension).

Flow cytometry surface staining pattern of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 μl reagent per million cells in 100 μl of cell suspension).