Exbio — Research products — Accessory reagents — Annexin binding — Annexin V Dyomics 647

Annexin V Dyomics 647

Regulatory status

RUO

Format

Dyomics 647

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

100 tests

EXB0023

In stock

200.00 USD

500 tests

EXB0030

In stock

670.00 USD

Variant

0.1 mg

11-261-C100

In stock

200.00 USD

0.1 mg

11-261-C100

In stock

670.00 USD

Contact distributor

Product details

Images

Application

FC (QC tested)

Description

Annexin V Dyomics 647 is intended for detection of early apoptotic cells by flow cytometry.

Contents

Annexin V Dyomics 647 - 1 vial (0.5 ml / 2.5 ml) of Ready-to-use solution. No reconstitution is necessary. Reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide. Sufficient for 100 / 500 tests.

Preparation

Bacterially expressed annexin V is purified to homogeneity (>95% by HPLC) and conjugated under optimum conditions. The reagent is free of unconjugated fluorochrome.

Formulation

Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide

Storage and handling

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Separation of Annexin positive Hoechst 34580 positive events (red-filled) from Annexin negative Hoechst 34580 negative events (black-dashed) in flow cytometry analysis (surface staining) of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 μl reagent per million cells in 100 μl of cell suspension).

Flow cytometry multicolor surface staining of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 μl reagent per million cells in 100 μl of cell suspension) and Hoechst 34580 viability stain.

Flow cytometry surface staining pattern of camptothecin induced apoptotic Jurkat cells stained using Annexin V Dyomics 647 (5 μl reagent per million cells in 100 μl of cell suspension).

Variant

100 tests

EXB0023

In stock

200.00 USD

500 tests

EXB0030

In stock

670.00 USD

Variant

0.1 mg

11-261-C100