Exbio — Clinical products — Reproductive immunology — Kits — SpermFlowEx Kit

SpermFlowEx Kit

Regulatory status

RUO

Reactivity

Human

Application

FC (QC tested)

Variant

25 tests

ED7079

In stock

549.00 USD

Variant

25 tests

ED7079

In stock

549.00 USD

Contact distributor

Product details

Images

References

IFU download

SDS download

Application

FC (QC tested)

Reactivity

Human

Description

The SpermFlowEx Kit is designed for analysis of following parameters in human semen using flow cytometry:
Sperm count
Leukocyte count
Sperm viability
Sperm acrosome integrity
Presence of intra-acrosomal protein in sperm
The analysis should be completed by the examination of sperm motility and morphology using a light microscope in accordance to WHO recommendation.

Contents

ED7079-1 Intra-acrosomal protein FITC – 1 vial containing 0.5 ml of mouse monoclonal antibody against intra-acrosomal protein, FITC labeled.
ED7079-2 CD45 PE-Cy5 – 1 vial containing 0.25 ml of mouse monoclonal antibody against CD45, PE-Cy5 labeled.
ED7079-3 Fluorescent Count Standard – 1 vial containing 2 ml of fluorescent beads, 1×10⁶ particles/ml
ED7079-4 Propidium Iodide – 1 vial containing 0.25 ml of propidium iodide solution.
ED7079-5 Permeabilizing Solution – 1 vial containing 25 ml of the reagent.

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Flow cytometry analysis of human sperma using Sperm FlowEx Kit. Upper part represents analysis of sperm count by using fluorescent count beads standard and surface staining of human semen with anti-CD45 PE-Cy™5. Lower part represents staining of human sperms with PI (left) and anti-Intra acrosomal protein (Hs-8) FITC (right).

Product specific references:

Pěknicová J, Chládek D, Hozák P (2005): Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol. 53(1): 42-9
PubMed

Krátká Z, Luxová Š, Vik V: Flow cytometry analysis of sperm quality in sub-fertile men. Ces Urol 2017; 21(3): 277-286

Further IFU language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
ED7079_TDS_v6_CZ.pdf
ED7079_TDS_v6_EN.pdf