Exbio — Clinical products — Premium antibodies (GMP) — Surface staining — Proaerolysin Alexa Fluor<sup>®</sup> 488

Proaerolysin Alexa Fluor^® 488

Regulatory status

RUO (GMP)

Antigen

Proaerolysin

Format

Alexa Fluor^® 488

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

1 ml

ED7751

In stock

396.00 USD

Variant

1 ml

ED7751

In stock

396.00 USD

Contact distributor

Product details

Images

References

SDS download

Specificity

Proaerolysin

Application

FC (QC tested)

Reactivity

Human

Description

Proaerolysin Alexa Fluor^® 488 is a fluorochrome-labeled recombinant protein single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine GPI deficiency in human clinical samples.
Proaerolysin Alexa Fluor^® 488 can be used in various multicolor combinations. Laboratory shall establish its performance characteristics in conjunction with other reagents in normal and pathological samples.

Contents

1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 200 tests

Concentration

0.075 mg/ml

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

Example of surface staining pattern using Proaerolysin Alexa Fluor® 488
Sample: WT Jurkat cells spiked with GPI-deficient Jurkat cells
Staining protocol: 10 μl reagent / million cells in 100 μl of cell suspension; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD3 positive living Jurkat cells displayed as Proaerolysin Alexa Fluor® 488 versus CD90 APC

Example of separation of positive (red-filled) and negative (black-dashed) events using Proaerolysin Alexa Fluor® 488
Sample: WT Jurkat cells spiked with GPI-deficient Jurkat cells
Staining protocol: 10 μl reagent / million cells in 100 μl of cell suspension; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: WT Jurkat cells
Negative events: GPI-deficient Jurkat cells

Example of surface staining pattern using Proaerolysin Alexa Fluor® 488
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)