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Exbio
—
Clinical products
—
Premium antibodies (GMP)
—
Surface staining
—
SingleFlowEx IgM PerCP-Cy™5.5
SingleFlowEx IgM PerCP-Cy™5.5
Regulatory status
RUO (GMP)
Antigen
IgM
Clone
CH2
Format
PerCP-Cy™5.5
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
1 ml
ED7786
Delivery 1 week
660.00 USD
Variant
1 ml
ED7786
Delivery 1 week
660.00 USD
Contact distributor
Product details
Images
SDS download
Isotype
Mouse IgG1
Specificity
IgM
Application
FC (QC tested)
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.020 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Example of surface staining pattern using SingleFlowEx IgM PerCP-Cy™5.5
Clone: CH2
Sample: pre-washed EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx IgM PerCP-Cy™5.5 versus CD19 Pacific Blue™
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx IgM PerCP-Cy™5.5
Clone: CH2
Sample: pre-washed EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: IgM positive CD19 positive lymphocytes
Negative events: Monocytes
Example of surface staining pattern using SingleFlowEx IgM PerCP-Cy™5.5
Clone: CH2
Sample: pre-washed EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
1 ml
ED7786
Delivery 1 week
660.00 USD
Variant
1 ml
ED7786
Delivery 1 week
660.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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