SingleFlowEx CD45RA Pacific Blue™

SingleFlowEx CD45RA Pacific Blue™
Regulatory status
RUO (GMP)
Antigen
CD45RA
Clone
MEM-56
Format
Pacific Blue™
Reactivity
Human
Application
Excitation laser
violet (405 nm)
Variant
1 ml
ED7713
Delivery 1 week
484.00 USD
Variant
1 ml
ED7713
Delivery 1 week
484.00 USD
Product details
Images
References
SDS download
Isotype
Mouse IgG2b
Specificity
CD45RA
Application
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.040 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
ED7713_FC_Dot-plot
Example of surface staining pattern using SingleFlowEx CD45RA Pacific Blue™
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD45RA Pacific Blue™ versus CD3 PE-Cy™7
ED7713_FC_Dot-plot_2
Example of surface staining pattern using SingleFlowEx CD45RA Pacific Blue™
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD45RA Pacific Blue™ versus CD45R0 FITC
ED7713_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD45RA Pacific Blue™
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD45RA positive CD3 positive lymphocytes
Negative events: Neutrophil granulocytes
ED7713_FC_Profil Značení
Example of surface staining pattern using SingleFlowEx CD45RA Pacific Blue™
Clone: MEM-56
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)

Product specific references:

Use of this product has not been published.
Variant
1 ml
ED7713
Delivery 1 week
484.00 USD
Variant
1 ml
ED7713
Delivery 1 week
484.00 USD

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