Exbio — Clinical products — Premium antibodies (GMP) — Surface staining — SingleFlowEx CD45R0 FITC

SingleFlowEx CD45R0 FITC

Regulatory status

RUO (GMP)

Antigen

CD45R0

Clone

UCHL1

Format

FITC

Reactivity

Human

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

1 ml

ED7569

Delivery 1 week

396.00 USD

Variant

1 ml

ED7569

Delivery 1 week

396.00 USD

Contact distributor

Product details

Images

References

SDS download

Isotype

Mouse IgG2a

Specificity

CD45R0

Application

FC (QC tested)

Reactivity

Human

Description

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Contents

1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.

Concentration

0.080 mg/ml

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Example of surface staining pattern using SingleFlowEx CD45R0 FITC
Clone: UCHL1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx CD45R0 FITC versus CD3 APC

Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx CD45R0 FITC
Clone: UCHL1
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; Lyse and Wash
Erythrocyte lysis: EXCELLYSE Easy (ED7066)
Positive events: CD45R0 positive CD3 positive lymphocytes
Negative events: Neutrophil granulocytes

Product specific references:

Kralickova P, Milota T, Litzman J, Malkusova I, Jilek D, Petanova J, Vidlakova J, Zimulova A, Fronkova E, Svaton M, Kanderova V, Bloomfeld M, Parackova Z, Klocperk A, Haviger J, Kalina T, Sediva A: CVID-associated tumors: Czech nationwide study focused on epidemiology, immunology, and genetic background in a cohort of patients with CVID. Frontiers in Immunology 2019, 9:3135