SingleFlowEx Perforin PE

SingleFlowEx Perforin PE
Regulatory status
RUO (GMP)
Antigen
Perforin
Clone
dG9
Format
PE
Reactivity
Human
Application
FC
Excitation laser
blue (488 nm)
Variant
1 ml
ED7308
Out of stock
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD
Product details
Images
Isotype
Mouse IgG2b kappa
Specificity
Perforin
Application
FC
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.100 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
The product is shipped with ice packs. Transport conditions, including short-term elevated temperature, do not impair product performance characteristics or do not affect stated product expiry date. Upon receipt, store and handle as directed.
Store at 2-8°C.
Keep away from sunlight.
Do not freeze.
Do not use after the expiry date stated on the product labels.
No reconstitution is necessary. Use the product directly from its original product container.
The product retains its performance characteristics until the expiry date when stored under stated conditions in its original product container.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
ED7308_FC_Dot-plot
Example of intracellular staining pattern using SingleFlowEx Perforin PE
Clone: dG9
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx Perforin PE versus CD56 APC
ED7308_FC_Histogram
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx Perforin PE
Clone: dG9
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397) Positive events: Perforin positive CD56 positive NK cells
Negative events: Neutrophil granulocytes
ED7308_FC_Profil Značení
Example of intracellular staining pattern using SingleFlowEx Perforin PE
Clone: dG9
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397)
Variant
1 ml
ED7308
Out of stock
440.00 USD
Variant
0.1 mg
11-261-C100
In stock
440.00 USD

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