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Exbio
—
Clinical products
—
Premium antibodies (GMP)
—
Intracellular staining
—
SingleFlowEx Perforin PE
SingleFlowEx Perforin PE
Regulatory status
RUO (GMP)
Antigen
Perforin
Clone
dG9
Format
PE
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
1 ml
ED7308
Delivery 1 week
440.00 USD
Variant
1 ml
ED7308
Delivery 1 week
440.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2b kappa
Specificity
Perforin
Application
FC (QC tested)
Reactivity
Human
Description
SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples.
SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
Contents
1 vial of monoclonal antibody reagent.
The content (1 ml) is sufficient for min. 100 tests.
Concentration
0.100 mg/ml
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s.
EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz.
EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Example of intracellular staining pattern using SingleFlowEx Perforin PE
Clone: dG9
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397)
Graphic display: gated CD45 positive lymphocytes displayed as SingleFlowEx Perforin PE versus CD56 APC
Example of separation of positive (red-filled) and negative (black-dashed) events using SingleFlowEx Perforin PE
Clone: dG9
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397) Positive events: Perforin positive CD56 positive NK cells
Negative events: Neutrophil granulocytes
Example of intracellular staining pattern using SingleFlowEx Perforin PE
Clone: dG9
Sample: EDTA-anticoagulated human peripheral blood, healthy donor
Staining protocol: 10 μl reagent / 100 μl blood sample; intracellular staining
Erythrocyte lysis and permeabilization: EXCELLYSE XPerm (ED7397)
Product specific references:
Use of this product has not been published.
Variant
1 ml
ED7308
Delivery 1 week
440.00 USD
Variant
1 ml
ED7308
Delivery 1 week
440.00 USD
Contact distributor
Datasheet download
SDS download
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EN
Bulk requests
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Related products
ED7397
EXCELLYSE XPerm
-134%
Clone
Reg. status
RUO
Application
FC
100 tests
200.00 USD
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